rhEPO对外伤性脑损伤大鼠血清S100B、IL-6表达的影响  

Effect of rhEPO on S100B protein and IL-6 expressions in serum of rats after traumatic brain injury

作　　者：卞晓星[1] 袁雪松[1] 戚传平[2] 

机构地区：[1]江苏大学附属武进医院神经外科,常州213002 [2]江苏大学附属武进医院中心实验室,常州213002

出　　处：《江苏医药》2009年第11期1306-1308,共3页Jiangsu Medical Journal

基　　金：常州市科技局社会发展基金资助项目(CS2007231)

摘　　要：目的探讨重组人促红细胞生成素(rhEPO)对大鼠外伤性脑损伤的神经保护机制。方法将60只健康成年雄性SD大鼠随机分成5组:1000 U/kg、3000 U/kg、5000 U/kg rhEPO治疗(A、B、C)组、胞二磷胆碱治疗(D)组和生理盐水(E)组。采用改进的Feeney法制作大鼠自由落体脑创伤模型。伤后6 h、24 h、3 d、5 d、7 d断尾采血,运用双抗体夹心ELISA方法检测各组治疗后血清中S100B蛋白和白细胞介素6(IL-6)的含量。结果与E组相比较,B和C组血清中S100B蛋白含量显著降低(P<0.01),rhEPO各治疗组血清中IL-6的含量均显著降低(P<0.01)。S100B蛋白下降程度与rhEPO剂量成正比,中等剂量rhEPO能更好降低血清中IL-6的含量。结论rhEPO可能通过抑制脑外伤后S100B和IL-6的合成与分泌,降低全身和局部的炎症反应程度,从而发挥神经保护作用。Objective To explore the neuroprotective mechanisms of recombinant humam erythropoietin（rhEPO） on the rats after traumatic brain injury. Methods Sixty healthy adult male SD rats were randomly dividied into 3 rhEPO-treated groups of 1000 U/kg （A）, 3000 U/kg（B）and 5000 U/kg, nicholin-treated group（D） and normal saline group（E） . The modified Feeneys traumatic brain injury model was made. Serum samples were collected at 6^th h, 24^th h, 3^rd, 5^th and 7^th d after trauma. The serum S100B protein and interleukirr6（IL-6） after treatment were detected with double antibody sandwich ELISA. Results compared with group E,the serum SI00B protein content was significantly lower in groups B and C （P〈0. 01）, and the serum IL-6 content was also significantly lower in 3 rhEPO-treated groups （P〈0.01）. The degree of decrease in serum S100B protein was positively correlated with the dosage of rhEPO. Medium dosage of rhEPO decreased the content of serum IL-6 more than the others. Conclusion Inhibition of the composition and secretion of S100B protein and IL-6 by EPO might be one of the mechanisms in decreasing inflammatory reaction systemically and locally and exerting a neuroprotective effect after traumatic brain injury.

关 键 词：促红细胞生成素 外伤性脑损伤 S100B 白细胞介素6 

分 类 号：R651[医药卫生—外科学]