梭梭小G蛋白基因HaRAN1克隆与表达分析  

Isolation and Characterization of HaRAN1 Encoding A Small G Protein in Haloxylon ammodendron

作　　者：史胜青[1] 张守攻[1] 肖文发[2] 齐力旺[1] 江泽平[1] 

机构地区：[1]中国林业科学研究院林业研究所,北京100091 [2]中国林业科学研究院森林生态环境与保护研究所,北京100091

出　　处：《西北植物学报》2009年第10期1939-1945,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国博士后科学基金(20070410579);国家863项目(2007AA021403);国家"十一五"科技支撑(2006BAD03A01-07)

摘　　要：采用cDNA-AFLP技术从梭梭中获得了一个干旱诱导的TDF序列,编码RAN基因的cDNA片段,利用5′-RACE和3′-RACE技术克隆到一个1083bp的全长基因,含有一个编码221个氨基酸的开放阅读框,具有结合GTP/GDP的4个保守结构域,属于RAN1亚家族,命名为HaRAN1。Blast分析表明,HaRAN1与单、双子叶植物(毛果杨、拟南芥、小麦、水稻)的一致性高达95%～96%以上;与哺乳动物(人类)和真菌(烟曲霉)的一致性高达88%以上;半定量RT-PCR分析显示,干旱、PEG和NaCl胁迫明显诱导HaRAN1基因的表达。结果表明,Ha-RAN1基因编码蛋白在物种间具有高度保守性,可能在梭梭适应极端干旱的沙漠生境中起重要作用。Haloxylon ammodendron（C.A.Mey.）Bunge,a stem-assimilating,xero-halophytic and halophytic desert shrub distributing naturally in the desert of north-west in China,where represents one of the harshest ecosystems on earth,combining drought,extreme temperatures and high salt.One TDF（transcript-derived fragment）encoding amino acids of RAN（small GTPase）protein,was obtained in H.ammodendron under drought stress by using cDNA-AFLP.Full-length gene HaRAN1,1 083 bp,was cloned by 5＇-RACE and 3＇-RACE,which contained an ORF encoding a 221 amino acids protein with four binding GTP/GDP conserved domains.Blast analysis showed that HaRAN1 have 95%～96% homologous to mono-and dico-tyledon（populus,arabidopsis,wheat and rice,et al.）,and 88% homologous to human and fungi,et al.Semi-quantitative RT-PCR showed the expression of HaRAN1 was induced significantly under drought,PEG and NaCl stress.The results suggested this protein encoded by HaRAN1 has very high homology among species and may play an important role in H.ammodendron response to extreme desert environments.

关 键 词：梭梭 RAN1 小G蛋白 基因克隆 

分 类 号：Q789[生物学—分子生物学]