植物无融合生殖的筛选和鉴定研究进展  

Research Progress in Techniques for Screening and Identifying Apomixis in Plants

作　　者：郝建华[1] 沈宗根[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500

出　　处：《西北植物学报》2009年第10期2128-2136,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省高校自然科学基金项目(08KJD210001);苏州市科技计划项目(YJG0911);常熟理工学院植物学重点建设学科;海藻遗传与种质创新实验室开放基金项目(2008001)

摘　　要：植物无融合生殖具有复杂的发育过程,与细胞学、遗传学、生物化学和分子生物学等相关的各种技术均被应用于植物无融合生殖的筛选和鉴定。本文结合新近发表的研究文献,介绍了常规的植物无融合生殖筛选和鉴定技术的新应用,评介了流式细胞种子筛选技术、胚珠整体透明技术、外源标记基因转入法等植物无融合生殖筛选和鉴定的新技术,并对各种筛选和鉴定技术的优势和不足进行了评述。Apomixis is now used synonymously with ＇agamospermy＇ meaning asexual reproduction by seeds or seed apomixis. It is a complex developmental process and various techniques involving cytological,genet- ic and histochemical and molecular examinations are used to screen for apomixes. In this paper,some of new applications of the commonly used techniques were introduced and some of the new techniques, such as flow cytometry seed screen （FCSS）,introduction of marker genes of antibiotic resistance, ovule clearing technology were presented. The advantages and disadvantages of various techniques were reviewed.

关 键 词：无融合生殖 流式细胞种子筛选 胚珠透明技术 繁殖方式 筛选方法 

分 类 号：Q944.47[生物学—植物学]