CHIRALPAK AD-RH手性柱直接拆分普罗帕酮对映体  

Direct Chiral Separation of Propafenone Enantiomers by RP-HPLC Using Chiralpak AD-RH

作　　者：罗月影 赵欣[1] 张乾[1] 杜培刚 刘燕[1] 赵娅[1] 萧溶 

机构地区：[1]水钢总医院临床药学实验室,贵州六盘水553028

出　　处：《贵阳医学院学报》2009年第5期536-539,共4页Journal of Guiyang Medical College

基　　金：贵州省卫生厅科学技术基金项目(gzwkj2008-1-055);六盘水市科技攻关计划项目(52020-2008-1-05)

摘　　要：目的:建立普罗帕酮对映体的反相高效液相拆分方法,为研究普罗帕酮手性异构体药代动力学提供依据。方法:在Chiralpak AD-RH柱上,考察流动相pH值、流速、乙腈比例、缓冲盐浓度、柱温对普罗帕酮手性异构体拆分的影响。结果:以20mmol/L磷酸盐缓冲液(pH8.5)-乙腈为流动相,流速0.5mmol/L,柱温25℃,在Chiralpak AD-RH手性柱上成功拆分普罗帕酮对映体。结论:普罗帕酮对映体在Chiralpak AD-RH手性柱上获得基线拆分。Objective ： To develop a RP-HPLC method for the enantiomeric separation of propafenone on a new chiral column Chiralpak AD-RH [ amylose tris （3,5-dimethylphenylcarbamate） coated on a 5 μm silica-gel substrate] for pharmaceutical study of propafenone, nebtods： Enantiomeric separation of propafenone was carried out with Chiralpak AD-RH, and the influences of mobile phase pH value, flow rate, ratio of acetonitrile, concentration of buffer, and column temperature on the chiral separation were observed. Results： Propafenone enantiomers were successfully separated on Chiralpak ADRH with a mobile phase consisting of 20 mmol/L phosphate buffer （pH8.5） and 1：1 （V/V） acetonitrile at a flow rate of 0.5 mmol/L. Conclusion ： A baseline separation of propafenone enantiomers was achieved on a Chiralpak AD-RH column.

关 键 词：普罗帕酮 手性拆分 直链淀粉类手性固定相 色谱法 高压液相 

分 类 号：R916.2[医药卫生—药学]