雷公藤甲素对哮喘小鼠气道重塑及转化生长因子-β_1表达的影响  

Effects of Triptolide on Airway Remodeling and TGF-β1 Expression in Asthmatic Mice

作　　者：吕志强[1] 陈茗[1] 江山平[1] 谭艳芳[1] 张蔚[1] 梁瑞韵[1] 李海刚[2] 

机构地区：[1]中山大学附属第二医院呼吸内科,广东广州510120 [2]中山大学附属第二医院病理科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2009年第5期537-542,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目社会发展计划项目(2005B30601001)

摘　　要：【目的】探讨雷公藤甲素对支气管哮喘(简称哮喘)小鼠气道重塑的影响。【方法】40只雌性SPF级BABL/c小鼠,随机数字表法分为4组,每组10只。A组为生理盐水对照组;B组为卵蛋白(OVA)哮喘组;C组为雷公藤甲素治疗组;D组为地塞米松治疗组。在末次激发24h后所有小鼠取左肺组织行苏木精-伊红(HE)染色,过碘酸-雪夫(PAS)染色,Masson三色染色以及抗转化生长因子β1(TGF-β1)抗体免疫组化染色。测定支气管管壁厚度(WAt/Pbm),支气管平滑肌厚度(WAm/Pbm)。取右肺组织行RT-PCR检测TGF-β1mRNA表达。【结果】B组小鼠支气管管壁厚度、杯状细胞百分数、气道平滑肌厚度、胶原纤维面积、TGF-β1的转录和表达水平均显著高于A组(P<0.01)。C组小鼠上述各指标依次均显著低于B组(P<0.05),D组小鼠上述指标显著低于B组(P<0.05)。但C组和D组上述各指标间差异无统计学意义(P>0.05)。肺组织的TGF-β1的蛋白表达水平与支气管的管壁厚度,平滑肌厚度,杯状细胞比,胶原纤维含量成正相关(相关系数分别为0.755、0.815、0.898、0.837;P<0.01)。【结论】雷公藤甲素可抑制BABL/c哮喘小鼠气道重塑的发生,其机制有可能是通过抑制TGF-β1的表达而实现的。【Objective】 To investigate the effect of triptolide on airway remodeling in asthmatic mice. 【Methods】 Forty female BABL / c mice were randomly divided into four groups with 10 mice in each group. ①Group A （control group）：mice were treated with saline; ②Group B（asthmatic group）：mice were sensitized and challenged with OVA; ③Group C（triptolide group）：mice were sensitized and challenged as asthmatic group, and treated with 40 μg triptolide before challenged; ④Group D（dexamethasone group）：mice were sensitized and challenged as above,and were given 2 mg dexamethasone by intraperitoneal injection before challenged. All mice were killed 24 h after the final OVA challenge. The left lung was isolated for pathological examination. Lung sections were stained with hematoxylin and eosin （HE）, PAS, Masson＇s trichrome and immunohistochemical staining for TGF-β1 were performed.The thickness of bronchial airway （WAt / Pbm）, the percentage of goblet cells, bronchial smooth muscle thickness （WAm / Pbm）, and the collagen deposition area were measured, and RT-PCR was performed to detect the mRNA expression of TGF- β1 from the right lung tissues. 【Results】 Bronchial airway thickness, the percentage of goblet cells, bronchial smooth muscle thickness, the collagen deposition area, TGF-β1 mRNA and the expression of TGF-β1 in group B were significantly higher than those of group A（P〈0.01）. The above index in group C and group D were significantly lower than those of group B（P〈0.05）. For index mentioned above, there were no significant differences between group C and group D（P〈0.05）. The changes of TGF-β1 expression were correlated significantly with bronchial airway thickness, the percentage of goblet cells, bronchial smooth muscle thickness, and the collagen deposition area（r = 0.755,0.815,0.898,0.837, P〈0.01）. 【Conclusions】 Triptolide can inhibit the development of airway remodeling in asthmatic mice,and the possible mechanism may be due to reduc

关 键 词：哮喘 气道重塑 小鼠 雷公藤甲素 

分 类 号：R562.25[医药卫生—呼吸系统]