脂质过氧化损伤对高脂血症大鼠重症急性胰腺炎的作用及机制  

Role of lipid peroxidation in the development of severe acute pancreatitis in rats with hyperlipemia

作　　者：林志辉[1] 黄胡萍[1] 

机构地区：[1]福建医科大学省立临床学院福建省立医院消化内科,福建省福州市350001

出　　处：《世界华人消化杂志》2009年第25期2561-2565,共5页World Chinese Journal of Digestology

摘　　要：目的:观察高脂血症对大鼠急性胰腺炎病情的影响,探讨脂质过氧化损伤在伴高脂血症重症急性胰腺炎(SAP)中的作用及其机制.方法:SD大鼠脂肪乳剂灌胃2wk建立高脂血症模型,逆行胰胆管注射3.5%牛磺胆酸钠诱发SAP模型.将大鼠50只随机分为4组:正常组(n=10);高脂血症对照组(HL组,n=10);SAP组(n=15);伴高脂血症SAP(HAP,n=15).检测血清淀粉酶(AMS)、甘油三酯(TG)及胆固醇(CH)水平,并检测血清及胰腺组织的丙二醛(MDA)、超氧化物歧化酶(SOD)、黄嘌呤氧化酶(XOD)、一氧化氮(NO),观察胰腺组织病理改变.结果:HAP组胰腺组织病理改变较SAP组严重;HAP组血清及胰腺组织MDA、XOD水平显著高于SAP组(血清:30.76±2.67nmol/mLvs23.14±3.42nmol/mL,55.72±10.49U/Lvs45.78±8.98U/L,均P<0.01;胰腺组织:4.33±0.48nmol/mgprotvs2.87±0.45nmol/mgprot,5.57±0.63U/gprotvs4.33±0.79U/gprot,均P<0.01);其血清及胰腺组织SOD、NO水平显著低于SAP组(血清:85.46±13.56U/mLvs97.16±13.77U/mL,31.72±10.50μmol/Lvs52.97±6.01μmol/L,均P<0.05;胰腺组织:22.65±3.85U/mgprotvs27.88±4.43U/mgprot,均P<0.01;1.09±0.21μmol/gprotvs1.48±0.40μmol/gprot,均P<0.05).结论:高脂血症可加SAP的胰腺病理改变;脂质过氧化损伤可能在高脂血症加重SAP的机制中发挥重要作用.AIM： To investigate the role of lipid peroxidation in the development of severe acute pancreafifis （SAP） in rats with hyperlipemia （HP）.METHODS： Sprague-Dawley （SD） rats were administered intragastrically a high-fat diet for two weeks to induce experimental HP. SAP was induced by retrograde injection of 3.5% sodium taurocholate into the pancreatic duct. Fifty male Sprague-Dawley rats were randomly divided into four groups： normal control group, HL group, SAP group and HAP （HL＋SAP） group. The levels of amylase （AMS）, triglyceride （TG） and cholesterol （CH） in the serum were mea- sured. The changes in the concentrations of malonaldehyde （MDA）, superoxide dismutase （SOD）, xanthine oxidase （XOD） and nitric oxide （NO） in the serum and pancreatic tissue were RESULTS： The pathological changes in the pan- creas were more severe in the HAP group than in the SAP group. The levels of MDA and XOD in the serum and pancreatic tissue were signifi- cantly higher in the HAP group than in the SAP group （serum： 30.76 ＋ 2.67 nmol/mL vs 23.14 ＋ 3.42 nmol/mL and 55.72 ＋ 10.49 U/L vs 45.78 ＋ 8.98 U/L, both P 〈 0.01; pancreatic tissue： 4.33 ＋ 0.48 nmol/mgprot vs 2.87 ＋ 0.45 nmol/mgprot and 5.57 ＋ 0.63 U/gprot vs 4.33 ＋ 0.79 U/gprot, both P 〈 0.01）, while the levels of SOD and NO in the serum and pancreatic tissue were signifi- cantly lower in the HAP group than in the SAP group （serum： 85.46 ＋ 13.56 U/mL vs 97.16 ＋ 13.77 U/mL and 31.72 ＋ 10.50 ~mol/L vs 52.97 ＋ 6.01 ganol/L, both P 〈 0.05; pancreatic tissue： 22.65 ＋ 3.85 U/mgprot vs 27.88 ＋ 4.43 U/mgprot and 1.09 ＋ 0.21 ~mol/gprot vs 1.48 ＋ 0.40 ~mol/ gprot, both P 〈 0.05）.CONCLUSION： Hyperlipemia can aggravate pancreatic pathological changes caused by se- vere acute pancreatitis in rats perhaps through induction of lipid peroxidation.

关 键 词：高脂血症 重症急性胰腺炎 脂质过氧化 氧自由基 

分 类 号：R589.2[医药卫生—内分泌] R576[医药卫生—内科学]