缬草波春对大鼠非炎症性结肠致敏模型的影响  

Valepotriates reduce colorectal sensitivity and inhibit colonic motility in rats with noninflammatory colonic hypersensitivity

作　　者：叶建明[1] 王钰[1] 汪晓珍[2] 何学斌[3] 蒋朋[3] 薛存宽[3] 胡品津[4] 

机构地区：[1]中山市人民医院消化内科,广东省中山市528403 [2]中山市人民医院儿科,广东省中山市528403 [3]华中科技大学同济医学院老年医学研究所,湖北省武汉市430077 [4]中山大学附属第一医院消化内科,广东省广州市510080

出　　处：《世界华人消化杂志》2009年第25期2616-2620,共5页World Chinese Journal of Digestology

基　　金：广东省中医药局建设中医药强省科研基金资助项目;No2007290~~

摘　　要：目的:探讨中药提取物缬草波春对大鼠非炎症性结肠致敏模型的影响.方法:将32只雄性Wistar大鼠随机分为4组(n=8):空白对照组、模型对照组、吗啡对照组及缬草波春药物实验组.正丁酸钠灌注直肠法制造大鼠非炎症性结肠致敏模型,缬草波春药物实验组给予缬草波春2.5mg/(kg-d),吗啡对照组吗啡0.4mg/(kg-d),生理盐水对照组和模型对照组等量生理盐水灌胃,共2wk.直肠扩张法检验大鼠直肠的敏感性,水囊通过压力感受器记录结肠用药前后直肠压力改变.分析各组直肠初始压力感觉与最大压力,结肠平均压力与最大耐受压力.结果:缬草波春组与模型对照组比较,在压力为0、1.33、2.66kPa时,直肠扩张积分差异无统计学意义,在压力为3.99、5.32、6.65、7.98kPa时,则有统计学意义(t=-2.50,-4.58,-3.00,-2.38,P<0.05或P<0.01),而与吗啡对照组比较,在上述压力下差异均无统计学意义;缬草波春组与模型对照组初始感觉压力比较明显升高,差异有显著意义(t=5.892,P<0.01),而与吗啡对照组比较,差异无显著意义;缬草波春组与模型对照组初始感觉压力和最大压力耐受值比较明显升高,差异有显著意义(t=5.892,5.075,均P<0.01),而与吗啡对照组上述两项指标差异无显著意义;缬草波春组与模型对照组和与吗啡组比较结肠运动平均压力与其运动最大压力明显降低,差异有显著意义(t=-6.583,-3.85,-3.698,-2.440,P<0.01或0.05).结论:缬草波春可降低大鼠非炎症性结肠致敏模型结、直肠的敏感性,抑制模型大鼠的结肠运动.AIM： To investigate the effects of valepotriates on colorectal visceral sensitivity and colonic motility in rats with noninflammatory visceral hypersensitivity.METHODS： Thirty-two male Wistar rats were randomly divided into four groups： normal control group, model control group, morphine treatment group and valepotriate treatment group. A model of noninflammatory visceral hypersensitivity was induced in rats by giving a rectal perfusion of sodium butyrate solution. Rats in the morphine treatment group and valepotriate treatment group were given morphine solution at a dose of 0.4 mg/（kg·d） and valepotriate solution at a dose of 2.5 mg/（kg·d）, respectively, while those in the normal control group and model control group were given equal volume of normal saline. Rectal distention test was performed to examine rectal sensitivity. A pressure sensor was used to record pressure changes in the colon and rectum before and after treatment. The pressures to produce initial sensation and maximum tolerated sensation in the rectum as well as the average and maximum pressures in the colon were compared among each group.RESULTS： No significant differences were noted in the scores for rectal distention between the valepotriate treatment group and model control group under a pressure of 0, 1.33 or 2.66 kPa. In contrast, significant differences were noted in the scores between the two groups under a pressure of 3.99, 5.32, 6.65 or 7.98 kPa （t = -2.50, 4.58, -3.00 and -2.38, respectively; all P 〈 0.05 or 0.01）. However, there were no significant differences in the scores between the two treatment groups under the above conditions. The pressures to produce initial sensation and maximum tolerated sensa- tion in the rectum were significantly higher in the valepotriate treatment group than in the model control group （t=5.892 and 5.075, respectively; both P 〈 0.01） though no significant differences were noted between the two treatment groups. The average and maximum pressures in the colon were significantly

关 键 词：缬草波春 缬草三酯 正丁酸钠 肠易激综合征 内脏高敏感 

分 类 号：R285.5[医药卫生—中药学]