新型壳聚糖止血粉止血作用的实验研究  

EXPERIMENTAL STUDY ON HEMOSTASIS OF A NEW CHITOSAN HEMOSTATIC POWDER

作　　者：尹刚[1] 侯春林[1] 刘万顺 韩宝芹 魏长征[1] 刘双利[1] 郭兴锋[1] 于兵[3] 

机构地区：[1]第二军医大学附属长征医院骨科,上海200003 [2]青岛博益特生物材料有限公司 [3]第二军医大学细胞生物学教研室

出　　处：《中国修复重建外科杂志》2009年第11期1347-1349,共3页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的探讨通过剂型改造和结构改变制备的一种新型壳聚糖止血粉的止血作用。方法取成年SD大鼠24只,雌雄不限,体重210～240g,制备肝中叶切除出血模型,根据切口使用止血材料不同随机分为3组(n=8),A1组300mg云南白药,B1组300mgpH6.5的新型壳聚糖止血粉,C1组300mgpH7.5的新型壳聚糖止血粉,记录出血时间及出血量。采用Lee等方法进行体外促凝血实验。分别取0.2mL终浓度为0.2mg/mL的云南白药(A2组)、pH6.5的新型壳聚糖止血粉(B2组)及pH7.5的新型壳聚糖止血粉(C2组)溶液,与2mL新西兰兔耳中央动脉血混匀,记录凝血时间。并取B2组及C2组壳聚糖血凝块进行扫描电镜观察。结果A1、B1及C1组出血时间分别为(292±31)、(261±23)、(224±28)s,出血量分别为(1.63±0.21)、(1.47±0.18)、(1.18±0.17)g;B1组及C1组出血时间及出血量与A1组比较,差异均有统计学意义(P<0.05);且C1组优于B1组(P<0.05)。A2、B2及C2组凝血时间分别为(653±41)、(255±20)、(202±11)s,B2、C2组与A2组比较,差异均有统计学意义(P<0.05);且C2组优于B2组(P<0.05)。扫描电镜下可见B2组及C2组血细胞均聚集于壳聚糖周围。结论pH7.5的新型壳聚糖止血粉具有良好的止血作用。Objective To study hemostasis of a new chitosan hemostatic powder. Methods Twenty-four adult SD rats were made the models of liver injury, male or female, and weighing 210-240 g. They were divided into three groups randomly （n=8） depending on different hemostatic powders. The incision of the liver was treated with 300 mg Yunnan baiyao （group A1）, chitosan hemostatic powder of pH6.5 （group B1） and pH7.5 （group C1）, respectively. The bleeding time and bleeding amount were recorded. In vitro, with the modified Ree-White method, 2 mL artery blood from New Zealand white rabbit was added into the 0.2 mL solution of Yunnan baiyao, chitosan hemostatic powder of pH6.5 and pH7.5 （concentration of 0.2 mg/mL）, respectively. The blood coagulation time was recorded. The chitosan blood clots of group B2 and group C2 were observed with scanning electron microscopy （SEM）. Results The bleeding time of group A1, group B1 and group C1 was （292 ± 31）, （261 ± 23）, and （224 ± 28） s, respectively, the bleeding amount was （1.63 ± 0.21）, （1.47 ± 0.18）, and （1.18 ± 0.17） g, respectively, showing statistically significant differences between groups B1, C1, and group A1 （P 〈 0.05）, between group C1 and group B1 （P 〈 0.05）. The blood clotting time of group A2, group B2, and group C2 was （653 ± 41）, （255 ± 20）, and （202 ± 11） s, respectively, showing statistically significant differences between groups B2, C2, and group A2 （P 〈 0.05）, between group C2 and group B2 （P 〈 0.05）. The SEM showed that the blood cells of group B2 and group C2 gathered around the chitosan. Conclusion Chitosan hemostatic powder ofpH7.5 has good hemostasis.

关 键 词：壳聚糖 止血粉 止血作用 

分 类 号：R318.08[医药卫生—生物医学工程] R619.1[医药卫生—基础医学]