奶牛乳房炎金黄色葡萄球菌毒力因子及荚膜基因的PCR检测  

PCR-based detection of genes encoding virulence determinants and capsular polysaccharides in Staphylococcus aureus from bovine subclinical mastitis cases

作　　者：迟佳琦[1] 李闰婷[2] 朱战波[1] 曹宏伟[2] 崔玉东[1,2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《中国预防兽医学报》2009年第11期860-863,909,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省科技攻关重大项目(GA02B501)

摘　　要：为研究金黄色葡萄球菌(S.aureus)的基因型特征,本实验利用聚合酶链式反(PCR)方法,对S.aureus的3个标准株和30个临床分离株的凝集因子A、凝固酶、溶血素等33种致病因子进行了检测。结果表明各分离株在毒力因子方面存在一定差异。这些差异的检测将为进一步研究S.aureus的致病性和有效防治提供参考。The present study was carried out to genotypically characterize Staphylococcus aureus strains isolated from bovine mastiffs cases. A total of 33 S. aureus strains were characterized by polymerase chain reaction （PCR） using primers that amplify 31 genes encoding coagulase （coa）, clumping factor （clfA）, hemolysin, capsular polysaccharides （CP）, and other virulence factors. The findings of the present studies would provide better understanding of the distribution of the prevalent S. aureus clones among bovine mastitis isolates, therefore aid in the development control measures against S. aureus infections in dairy herds.

关 键 词：奶牛乳房炎 金黄色葡萄球菌 毒力因子 荚膜血清型 PCR检测 

分 类 号：S855.11[农业科学—临床兽医学] Q343.12[农业科学—兽医学]