捻转血矛线虫Hc38基因DNA疫苗对绵羊免疫保护性效果评价  

Protective effects of Hc38 DNA vaccines against Haemonchus contortus in sheep

作　　者：郑金海[1] 段正秀[2] 赵军明[3] 薄新文[1] 钟发刚[1] 张慧[1] 

机构地区：[1]新疆兵团绵羊繁育生物技术重点实验室,新疆石河子832000 [2]石河子大学生命科技学院,新疆石河子832000 [3]中国热带农业科学院科技信息所,海南儋洲571737

出　　处：《中国预防兽医学报》2009年第11期898-901,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：新疆兵团科技局社会发展项目(2006GG31)

摘　　要：为了研究基因疫苗对绵羊的免疫保护效果,本研究构建了捻转血矛线虫(H.contortus)Hc38基因DNA疫苗。将H.contortus Hc38基因保守结构域克隆到真核表达载体pcDNA3.1中,免疫鼠8d后用RT-PCR检测到该疫苗在鼠肌肉组织中进行了转录。将纯化的DNA疫苗免疫绵羊后,用western blot和ELISA方法检测疫苗在绵羊体内的翻译和诱导IgG的产生。二免后2周用10000条H.contortus第3期幼虫攻击实验动物,检测绵羊粪便虫卵排出、成虫数量等免疫保护性指标。该H.contortus Hc38 DNA疫苗与对照组比较,免疫组绵羊排出虫卵减少66.6%、成虫减少33.1%。特别值得注意的是免疫组的静脉注射方式产生抗体最高,相应羊的虫卵数和成虫数低。本实验证明Hc38基因DNA疫苗对绵羊虫卵及成虫发育具有明显的抑制作用。To study protective effects of Hc38 DNA vaccines against Haemonchus contortus in sheep, we constructed DNA vaccines containing conserved Hc38 protein domain. The Hc38 gene was inserted into pcDNA3.1 and administered to mice. Transcriptions of the vaccines were confirmed by RT-PCR in mouse muscles. Sheep were immunized with DNA vaccine and antibody （IgG） production was analyzed by western blot and ELISA. After 2 weeks, all sheep were challenged by 10000 H. contortus L3 strain. The results showed that EPG （eggs per gram feces） and worm burdens in immunized sheep reduced by 66.6 % and 33.1%, compared to the control group. Vaccination by intravenous injection induced highest antibody level and resulted in lowest EPG and worm burdens in sheep.

关 键 词：绵羊 捻转血矛线虫 Hc38 PCDNA3.1 DNA疫苗 

分 类 号：S852.7[农业科学—基础兽医学]