利用白色杜洛克×二花脸资源家系定位影响猪肉硬实度的QTL  

Genome-wide Identification of Quantitative Trait Loci for Pork Firmness in a Large Scale White Duroc × Chinese Erhualian Resource Population

作　　者：段艳宇[1] 周利华[1] 麻骏武[1] 郭蓓丽[1] 黄卫兵[1] 乔瑞敏[1] 韩鹏飞[1] 姚飞[1] 黄路生[1] 

机构地区：[1]江西农业大学生物技术国家重点实验室培育基地,南昌330045

出　　处：《畜牧兽医学报》2009年第11期1582-1587,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家杰出青年基金项目(30425045)资助

摘　　要：为定位影响猪背最长肌和半膜肌硬实度的数量性状基因座(QTL),本研究测定了白色杜洛克×二花脸资源家系中490头F2代猪的背最长肌和半膜肌硬实度,检测了3代个体在19条染色体上194个微卫星的基因型,并据此进行了QTL定位分析。共检测到9个QTLs,包括影响背最长肌硬实度的4个QTLs,分别位于SSC1、SSC2、SSC7和SSC10,其中SSC1和SSC7的QTLs达到1%基因组显著水平;影响半膜肌硬实度的5个QTLs分别位于SSC1、SSC7、SSC10、SSC12和SSC14,其中SSC7和SSC14的QTLs分别达到5%和1%基因组显著水平。To identify quantitative trait loci（QTLs）for firmness of M.Longissimus dorsi（LD） and M.Semimembranous（SM） in pigs,the related traits were measured in 490 F2 animals from a White Duroc × Erhualian resource population.A whole genome scan was performed with 194 microsatellites on 19 porcine chromosomes,and the QTL analysis was performed based on a least-square regression method.A total of 9 QTLs were identified,including 4 QTLs for firmness of LD on SSC1,SSC2,SSC7 and SSC10,those on SSC1 and SSC7 reached the 1% genome-wide significant level.Five QTLs for firmness of SM were detected on SSC1,SSC7,SSC10,SSC12 and SSC14,the QTLs on SSC7 and SSC14 showed 5% and 1% genome-wide significant effects respectively.

关 键 词：数量性状基因座 肌肉硬实度 猪 

分 类 号：S828[农业科学—畜牧学] Q343.17[农业科学—畜牧兽医]