不同来源禽网状内皮增生病病毒株的致病性比较  

Comparision of Pathogenicity of Six Different Reticuloendotheliosis Virus Strains to 1-day-old SPF Chickens

作　　者：孙淑红[1] 崔治中[1] 王辉[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2009年第11期1658-1661,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：博士点基金(20040434002#);农业部十一五支撑计划(2006BAD06A08)

摘　　要：本研究旨在探讨不同来源的禽网状内皮增生病病毒(REV)对1日龄SPF鸡的致病性。用包括分子克隆化病毒REV-C99在内的不同来源的6个REV株人工感染1日龄SPF鸡,以禽流感病毒灭活疫苗(H9-AIV、H5-AIV)与新城疫病毒灭活疫苗(NDV)免疫后HI抗体滴度的测定结果为指标,比较不同REV株的致病性。结果表明,分别用3 000TCID50.只-1的剂量人工感染1日龄SPF鸡,6个REV感染组与对照组相比,H9-AIV、H5-AIV与NDV免疫后4、5与6周的HI抗体水平均极显著降低(P<0.01),但6个REV感染组之间差异不显著(P>0.05)。研究结果显示,不同来源REV株感染1日龄SPF鸡后均造成生长迟缓,并对体液免疫反应有明显的抑制作用,同时,这也揭示出REV感染可能是免疫失败的重要原因之一。This experiment was conducted to investigate the pathogenicity of different Reticuloendotheliosis virus（REV） strains on 1-day-old SPF chickens.1-day-old SPF chickens were inoculated with six REV strains including REV-C99,a cloned strain.The pathogenicity of REV from different strains was evaluated by the measurement of growing rate of chicks and the antibody titers to the Newcastle disease virus（NDV） and Avian influenza virus（H9-AIV and H5-AIV） after vaccination.The results showed that REV challenge at a dose of 3 000 TCID50/bird resulted in the significantly（P0.05） suppressed HI titers of NDV,AIV（H5-,H9-） at the 4-,5-and 6-week after vaccination compared to control group,whereas there was no significant difference between the 6 REV strains.The results indicated that REV challenge at 1-day of age would retard the growth of chicks and suppressed the humoral immunity.The result suggests that REV challenge is one of the important reasons of immune failure.

关 键 词：禽网状内皮增生病病毒 HI 免疫抑制 致病性 

分 类 号：S852.659.3[农业科学—基础兽医学]