木黄酮对小鼠胰岛β细胞上电压依赖性钾电流的影响  

Effect of genistein on voltage-gated potassium current in mouse pancreatic islet-cells

作　　者：毕媛[1] 赵玉峰[2] 刘亚莉[2] 朱运龙[2] 王捷[1] 

机构地区：[1]广州军区广州总医院医学实验科,广东广州510010 [2]第四军医大学基础部生理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2009年第21期2356-2358,共3页Journal of the Fourth Military Medical University

摘　　要：目的:研究酪氨酸激酶抑制剂木黄铜活性对胰岛β细胞中电压依赖性钾电流的影响,探讨酪氨酸激酶在胰岛β细胞中的作用.方法:原代培养小鼠胰岛β细胞,经0.1mmol/L酪氨酸激酶抑制剂木黄酮急性处理处理或者慢性处理12h后,运用全细胞电流记录的方法观察电压依赖性钾电流的改变.结果:木黄酮急性处理可显著抑制小鼠胰岛β细胞的电压依赖性钾电流.木黄酮处理12h后,小鼠胰岛β细胞的电压依赖性钾电流较对照组明显减小,降低为对照组的59.3%8.5%(P<0.01,n=6).结论:木黄酮抑制小鼠胰岛β细胞中电压依赖性钾电流,提示酪氨酸激酶活性抑制在胰岛β细胞功能损害中具有重要作用.AIM：To determine the effect of inactivation of tyrosine kinases on voltage-gated potassium channels in pancreatic-cells and to evaluate the function of tyrosine kinases in pancreatic-cells. METHODS：Primarily cultured mouse pancreatic islet-cells were treated by 0.1 mmol/L genistein acutely or for 12 hours. Voltage-gated potassium currents were recorded with patch clamp techniques in the configuration of perforated whole-cell recording. RESULTS：The whole-cell voltage-gated potassium currents were significantly diminishes by acute treatment with genistein. After treatment by genistein for 12 hours, the whole- cell voltage-gated potassium currents were also decreased as compared to the control group. CONCLUSION：Genistein treatment decreases the current of voltage-gated potassium currents in mouse pancreatic-cells, which suggests that inhibition of tyrosine kinases activity plays an important role in the dysfunction of pancreatic- cells.

关 键 词：胰岛Β细胞 木黄酮 电压依赖性钾通道 

分 类 号：R335[医药卫生—人体生理学]