运动对糖尿病小鼠骨骼肌基因表达影响的生物信息学分析  

Bioinformatic Analysis of Gene Expression in Skeletal Muscle of Diabetic Mice after Physical Training

作　　者：王妹[1] 吴飞珍[2] 王宣春[1] 胡仁明[1] 

机构地区：[1]复旦大学附属华山医院内分泌科,复旦大学内分泌糖尿病研究所,上海200040 [2]上海大学电子生物技术研究中心,上海200072

出　　处：《中国运动医学杂志》2009年第6期670-674,共5页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金面上项目(30770854;30670999)

摘　　要：目的:探讨运动对链脲佐霉素诱导的糖尿病小鼠骨骼肌基因表达谱的影响及其生物代谢过程变化。方法:从GEO数据库下载Lehti等提交的小鼠骨骼肌基因芯片数据,应用生物信息学方法筛选差异表达基因,并对这些差异表达基因的基因本体(GO)和日本京都基因和基因组百科全书代谢通路数据库(KEGG)的注释进行功能富集分析。结果:与糖尿病非运动组小鼠相比,糖尿病运动组小鼠骨骼肌存在253个差异表达基因,这些基因主要富集于三条KEGG代谢通路和三个基因本体生物过程,代谢通路包括粘着斑通路、细胞外基质受体交互通路和细胞通讯;基因本体生物过程包括己糖代谢过程、单糖代谢过程和细胞成熟。结果提示运动影响糖尿病小鼠骨骼肌的粘着斑通路、细胞外基质受体交互通路和细胞通讯三条通路,从而改善糖代谢过程。Objective To address the effects of physical training on gene expression profile and biological metabolism process in mouse skeletal muscle with streptozotocin-induced diabetes. Methods We downloaded the gene chip datasets of mouse skeletal muscle from GEO databases （submitted by Lehti TM,et al.）, and then identified differential expression genes using bioinformatic approach. Furthermore,we applied enrichment analysis to the gene ontology（GO）and Kyoto Encyclopedia of Genes and Genomes（KEGG） of these differential expression genes. Results As compared with the untrained diabetic group,253 differential expression genes in skeletal muscle of training diabetic group were found. They mainly enriched in three KEGG pathways,including focal adhesion pathway, ECM-receptor interaction and cell communication,and three biological processes of GO,including hexose metabolic process,monosaccharide metabolic process,and cell maturation. These results suggested that physical training may affect the focal adhesion pathway, ECM-receptor interaction and cell communication,improving the glucose metabolism process in diabetic mouce.

关 键 词：糖尿病 运动 生物信息学 基因表达 

分 类 号：R587.1[医药卫生—内分泌] R734.2[医药卫生—内科学]