旋覆代赭汤对胃窦平滑肌细胞收缩和钙离子浓度的影响  

Effects of Xuanfu Daizhe Decoction on cell contraction and intracellular calcium concentration in rat gastric antral smooth muscle cells

作　　者：时昭红[1] 陶春晖[2] 石拓[1] 林丽莉[2] 王湘宁[2] 韦秀明[2] 

机构地区：[1]武汉市第一医院消化内科,湖北省武汉市430022 [2]湖北中医学院中医系,湖北省武汉市430061

出　　处：《世界华人消化杂志》2009年第28期2936-2939,共4页World Chinese Journal of Digestology

基　　金：武汉市卫生局临床重点学科研究基金资助项目;武卫No.[2004]241~~

摘　　要：目的:观察不同浓度旋覆代赭汤含药血清对大鼠胃窦平滑肌细胞(SMC)Ca2+浓度的影响,并探讨其对胃SMC收缩力作用机制.方法:分离大鼠胃窦SMC,加入不同浓度(5%、10%、20%)含药血清和胃动素,观察含药血清对胃窦SMC收缩作用,CPC法检测细胞Ca2+总浓度变化.结果:5%、10%、20%旋覆代赭汤含药血清刺激胃窦SMC,随剂量的增加细胞收缩活动逐渐增强(P<0.05).细胞收缩后,各剂量组与无钙缓冲液组的细胞内钙离子总浓度相比较无明显差异.结论:旋覆代赭汤可使胃窦SMC明显收缩,其机制可能是与IP3R使细胞内质网释放Ca2+引起胃窦SMCCa2+升高有关.AIM：To observe the effects of different concentrations of serum from rats medicated with Xuanfu Daizhe Decoction on cell contraction and intracellular calcium （Ca^2＋） concentration in rat gastric antral smooth muscle cells （SMCs）.METHODS：After single SMCs were isolated and cultured,motilin or different concentrations （5%,10% or 20%） of serum from rats medicated with XuanFu Daizhe Decoction were added,respectively.The contraction of SMCs was then determined,and intracellular Ca^2＋ concentration was measured using the o-cresolphthalein complexone （CPC） method.RESULTS： Serum from rats medicated with XuanFu Daizhe Decoction could significantly stimulate the contraction of gastric antral SMCs in a concentration-dependent manner （P 〈 0.05）. After cell contraction was induced, the total intracellular calcium concentration showed no significant difference among each serum treatment group and control group. CONCLUSION： Serum from rats medicated with XuanFu Daizhe Decoction can induce obvious contraction of gastric antral SMCs in rats perhaps via a mechanism involving activation of inositol trisphosphate receptor to stimulate the release of Ca^2＋ from intracellular stores.

关 键 词：旋覆代赭汤 含药血清 胃窦平滑肌细胞 钙离子 

分 类 号：R285.5[医药卫生—中药学]