CMO与BADH双基因表达载体构建及转化羊草的研究  

Construction of CMO-BADH Doublegene Expression Vector and Its Expression in Chinese Wildrye

作　　者：曲同宝[1] 邓川[2] 王丕武[1] 

机构地区：[1]吉林农业大学,长春130118 [2]吉林大学,长春130062

出　　处：《中国生物工程杂志》2009年第11期48-52,共5页China Biotechnology

基　　金：吉林省教育厅(吉科合字[2008]第315号)

摘　　要：羊草是分布于我国北方地区重要的禾本科牧草,为了保护羊草资源和培育羊草新品种,利用基因枪技术进行了CMO和BADH双基因转化羊草的研究。首先,以1301质粒为基础,构建了均由35S启动子驱动的无抗生素选择标记CMO基因和BADH基因的植物双基因表达载体pCAMBIA-1301-CMO-BADH;再通过基因枪技术将其导入羊草中,PCR扩增后电泳检测及Southern杂交分析表明,外源基因已整合到受体植物基因组中并正常表达。在高浓度混合盐和高pH值胁迫下,转双基因植株甜菜碱含量高于对照植株。Chinese wildrye （Leymus chinensis） is an important grass of the Gramineae family, which widely grows in the natural grassland of Northern China. In order to protect the resource of Chinese wildrye and culture new variety, the gene of CMO and BADH being transfered to Chinese wildrye by particle accelerator. A doublegene plant expression vector, pCAMBIA-1301-CMO-BADH were reconstructed based on PC1301 plasmid by CMO and BADH which were both driven by 35S promoter; It was transferred to Chinese wildrye by genegun, detected by PCR analysis and Sorthern blotting respectively which conclude that the foreign gene has consistented with the recipient plants＇ genome and expresses normally. The analysis of the content of betaine showed that it was higher in the doublegene vector transgenic plants than the blank plants.

关 键 词：羊草 CMO BADH 基因枪 甜菜碱 

分 类 号：Q78[生物学—分子生物学]