质粒DNA提取的简便方法  

An Improvement Method on Plasmid Extraction

作　　者：陈绍兴[1] 李沁[1] 董艳[1] 杨权芬[1] 陈娅[1] 钱丽芳[1] 

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661100

出　　处：《天津农业科学》2009年第5期4-5,共2页Tianjin Agricultural Sciences

基　　金：红河学院生物化学与分子生物学重点学科建设项目(071010);红河学院校级课题项目(XJ1Y0802);云南省教育厅科学研究基金(09Y0413)

摘　　要：以质粒pBR322为对象,在经典的质粒提取方法的基础上进行了一些改进,将酚氯仿异戊醇抽提和氯仿异戊醇抽提省去。比较了简便方法和经典方法提取质粒的效果,结果显示:两种方法均能够提取出质粒DNA,RNA的量并没有比经典方法提出来的多;简便方法抽取的质粒,蛋白含量稍稍多于后者;简便方法节省了大约一半的提取时间及大量的药品,并且减少了有害的化学物质对人体的危害,是一种经济实用的方法,完全可以满足一般实验需要。Plasmid pBR322 was used in this study and some improvement wew made based on the classical extraction method. The step of extracting by phenol chloroform isoamyl alcohol and chloroform isoamyl alcohol was omitted. Comparing the result of this two extraction method, it can find that the two methods both can get plasmid DNA and the quantity of RNA was no more than it by using the classical way. After omitting the two extraction method, the amount of protein is a little more than classical method. On one hand it will save about a half time, on the other hand it also can save plenty of physic. It will decrease the harm to human. This is an economical and convenient method and it can absolutely match the need of general experiment.

关 键 词：质粒DNA 提取 方法 PBR322 

分 类 号：Q78[生物学—分子生物学]