瘤果黑种草子挥发油的化学成分分析及百里醌的定量  

Analysis on chemical components of volatile oil and determination of thymoquinone from seed of nigella glandulifera

作　　者：耿东升[1] 张淑锋[2] 兰建国[1] 

机构地区：[1]新疆军区联勤部药品仪器检验所,新疆乌鲁木齐830063 [2]新疆医科大学,新疆乌鲁木齐830054

出　　处：《中国中药杂志》2009年第22期2887-2890,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30960512)

摘　　要：目的:分析瘤果黑种草子Nigella glandulifera Freyn(NG)挥发油化学成分,与国外果黑种草子N.sativa(NS)、黑种草子N.damascena(ND)比较,对水蒸气蒸馏法(hydrodistillation,HD)提取NG挥发油中百里醌定量。方法:采用HD与SFE-CO2萃取从NG提取挥发油,用GC-MS对化学成分分析,采用峰面积归一化法测定各成分相对百分含量,与NS,ND挥发油比较;用气相色谱外标一点法对挥发油中百里醌定量。结果:3个种属挥发性成分比较,NG,NS主要成分为间-伞花烃,分别是33.75%,61.48%,ND为β-榄香烯73.24%,NG,NS,ND百里醌相对百分含量分别为3.73%(HD),3.80%(HD),0.08%(HD),SFE-CO2主要成分为亚油酸(66.36%),间-伞花烃较低(0.93%);NG挥发油百里醌定量1.58%。结论:NG挥发性成分与NS,ND有较大差异。Objective： To analysis chemical components of volatile oil from the seed of Nigella glandulifera （ NG）, comparing them with those from the seed of foreign N. sativa （NS） and N. damascene （ ND）, and to quantify thymoquinone in the volatile oil extracted by hydrodistillation （HD） from the seed of NG. Method： The volatile oil was extracted by supercritical CO2 extraction （SFE- CO2 ） and HD from the seed of NG and its chemical components was analysed by GC-MS, the relative percentage of components were determined by peak aera normalization method and compare with those of the seed of NS and ND. The content of the thymoquinone in the volatile oil was determined by one point external standard method. Result： In terms of the volatile compounds, p-cymene is the major component of NG and NS, their relative percentage contents are 33.75% and 61.48%, respectively. β-Elemene is the major component of ND, its relative percentage content is 73.24%. The relative percentage contents of thymoquinone are 3.73% （HD）, 3.80% and 0. 08% in NG, NS and ND, respectively. Linoleic acid is a major component of volatile oil by SFE-CO2 in NG, but its content ofp-cymene is lower. The absolute percentage content of thymoquinone is 1.58% by HD in volatile oil of NG. Conclusion： There are comparatively large differences of volatile components in NG, NS and ND.

关 键 词：瘤果黑种草子 挥发油 化学成分 百里醌 

分 类 号：R284.1[医药卫生—中药学] R284[医药卫生—中医学]