RP-HPLC同时测定天麻中4种成分的含量  

Simultaneous determination of four components in Gastrodia elata by RP-HPLC

作　　者：闫宝庆[1] 张晖芬[1] 逄楠楠[1] 陈晓辉[1] 于治国[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2009年第22期2903-2906,共4页China Journal of Chinese Materia Medica

摘　　要：目的：建立RP-HPLC同时测定天麻药材中天麻素、腺苷、对羟基苯甲醇和对羟基苯甲醛的含量。方法：采用Kromasil C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.1%醋酸水溶液为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长270 nm,柱温35℃。结果：天麻素、腺苷、对羟基苯甲醇和对羟基苯甲醛分别在19.1-383（r=0.999 9）,0.620-12.4（r=0.999 9）,2.45-49.0（r=0.999 9）,0.280-5.63 mg.L-1（r=0.999 6）与峰面积线性关系良好;平均加样回收率（n=9）均在96.7%-97.7%,RSD均小于1.6%。结论：本法准确可靠,分离度好,适用于天麻药材中天麻素、腺苷、对羟基苯甲醇和对羟基苯甲醛的含量测定。Objective： To develop a reversed-phase HPLC method for simultaneous determination of gastrodin, adenosin, 4- hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia. Method： A Kromasil C18 column （4. 6 mm × 250 mm, 5 μm） was used with a methanol-water-0. 1% acetic acid gradient elution system. The eluates were detected at 270 nm, the flow rate was 1.0 mL· min^-1 and the column temperature was 35℃. Result： The linear range of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde were 19. 1-383 （r =0. 999 9）, 0. 620-12.4（r =0. 999 9）, 2.45-49.0 （r =0. 999 9）, 0. 280-5.63 mg · L^-1 （r =0. 999 6）, respectively. The average recoveries （ n = 9） of the four components were 96.7% -97.7% , RSD 〈 1.6%. Conclusion： The method is accurate, sensitive and reliable for determination of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.

关 键 词：天麻 RP-HPLC 同时含量测定 

分 类 号：R284.1[医药卫生—中药学]