姜黄素固体分散体对大鼠胃溃疡的疗效研究  

Pharmacological researches of curcumin solid dispersions on experimental gastric ulcer

作　　者：梅雪婷[1] 许东晖[1] 王胜[1] 许实波[1] 

机构地区：[1]中山大学生命科学学院中药与海洋药物研究室,广东广州510275

出　　处：《中国中药杂志》2009年第22期2920-2923,共4页China Journal of Chinese Materia Medica

基　　金：教育部2004年度"新世纪优秀人才支持计划"(NCET-04-0808)

摘　　要：目的:研究姜黄素固体分散体(SDs,姜黄素:聚乙烯吡咯烷酮PVP k30质量比1∶8)对大鼠胃溃疡的作用。方法:大鼠设为模型组、姜黄素SDs低、中、高剂量(含姜黄素10,30,90 mg.kg-1)组,阳性对照雷尼替丁(27 mg.kg-1)组。每组10只。乙酸致大鼠胃溃疡模型,连续口服给药14 d后,取血测定血清NO、血浆ET,测定胃溃疡指数;制备幽门结扎致大鼠胃溃疡模型,给药3 d后禁食36 h麻醉条件下施幽门结扎术,16 h后解剖收集胃液,测定胃液量、胃蛋白酶活性、溃疡指数;小鼠口服给药3 d后禁食24 h,第5天皮下注射利血平10 mg.kg-1制备胃溃疡模型,6 h后处死测定胃溃疡指数。结果:乙酸致胃溃疡实验表明,与模型组溃疡指数5.87±0.48,血清NO(23.63±5.73)mmol.L-1相比,口服姜黄素SDs中、高剂量组大鼠溃疡指数显著降低,分别为4.59±0.96,3.33±0.93(P<0.01),大鼠血清NO水平明显升高,分别为(29.75±5.90)mmol.L-1(P<0.05),(39.63±12.73)mmol.L-1(P<0.01);与模型组血浆ET(163.65±63.84)ng.L-1相比,姜黄素SDs高剂量明显降低大鼠的血浆ET水平(104.22±63.84)ng.L-1(P<0.05)。幽门结扎胃溃疡实验表明:与模型组溃疡指数相比,姜黄素SDs高剂量能够显著降低大鼠溃疡指数(P<0.01);与模型组胃液量、胃酸分泌以及胃蛋白酶活性相比,姜黄素SDs中、高剂量能够明显降低大鼠胃液量,分别为(12.68±1.46)mL(P<0.05),(9.99±0.79)mL(P<0.01),能够明显降低大鼠胃酸分泌(77.62±8.34)mmol.L-1(P<0.05),(65.77±8.19)mmol.L-1(P<0.01),能够明显抑制大鼠胃蛋白酶活性(358.13±37.44)U.mL-1(P<0.05),(292.13±41.93)U.mL-1(P<0.01)。利血平诱导胃溃疡模型实验表明:与模型组溃疡指数相比,姜黄素SDs中、高剂量组能够显著降低利血平诱导胃溃疡的溃疡指数,分别为3.88±0.40,3.03±0.64(P<0.01)。结论:通过多种实验性胃溃疡模型证明:姜黄素SDs具有明显的抗胃溃疡作用,通过抑制胃酸的分泌、降低胃液的酸度、抑制胃蛋白酶活性,促Objective： To research the pharmacological action of curcumin solid dispersions （SDs, curcumin and polyvinylpyrrolidone （PVP） k30 in the ratio of 1： 8） was investigated on experimental gastric ulcer in rats and mice. Method： Animals were randomly divided into several experimental groups. Each group consisted of 10 animals. The control group received PVP vehicle （720 mg·kg^-1 , po） throughout the course of the experiments. The treatment groups received different doses of curcumin SDs （equivalent to curcumin 10, 30 and 90 mg ·kg^-1 , po）, and ranitidine （27 mg ·kg^-1 , po） was used as the positive control. In acetic acid-induced gastric ulcers model, serum NO, plasma ET and gastric ulcer indexes of rats were measured after oral administration for 14 d. In rat ulcer model induced by pylorus-ligature, gastric volume pepsin and gastric ulcer indexes of rats were measured after oral administration for 3 d and pyloms-ligature inducement for 16 h. Gastric ulcer indexes of mice were measurement after oral administration for 3 d and subcutaneous injection reserpine 10 mg ·kg^-1. Result： The results showed that curcumin SDs （equivalent to curcumin 30, and 90 mg ·kg^-1 , po） could reduce the ulcer indexes 4. 59 ± 0. 96 and 3. 33 ± 0. 93 （P 〈 0. 01 ）, and increase serum NO level （29. 7± 5. 90） mmol· L^-1 （p 〈 0. 05） and （39. 63 ±12. 73） mmol · L^-1 （P 〈0. 01 ）, compared to gastric index 5. 87 ±0. 48 and NO level （23.63 ±5.73） mmol· L^-1 in control group. Compared to plasma ET （163. 65 ±63.84） ng · L^-1 in control group, curcumin SDs （equivalent to 90 mg· kg^-1 , po） could decrease plasma ET level （ 104. 22 ±63. 84） ng· L-1 （P 〈0. 05）. Compared to gastric ulcer indexes 4. 25 ±0. 71 of control group in rat pylorus-ligature model, curcumin SDs （ equivalent to curcumin 90 mg · kg^-1 ） could reduce gastric ulcer to 2. 38 ± 0. 74 （ P 〈 0. 01 ）. Compared to gastric volume （ 14. 61± 1.80） mL, acidity of gastric juic

关 键 词：姜黄素 聚乙烯吡咯烷酮 固体分散体 胃溃疡 

分 类 号：R285.5[医药卫生—中药学]