制川乌总碱与白芍总苷、白芍多糖配伍治疗类风湿性关节炎大鼠的作用机制研究  

Mechanism study of action on compatible using of total alkaloids of Radix Aconiti Praeparata and total glycosides or polysaccharides of Radix Paeoniae Alba therapy on rheumatoid arthritis in rats

作　　者：李晋奇[1,2] 彭成[1] 姬洁莹[3] 

机构地区：[1]成都中医药大学,四川成都610075 [2]四川省医学科学院四川省人民医院,四川成都610072 [3]山西生物应用职业技术学院,山西太原030031

出　　处：《中国中药杂志》2009年第22期2937-2942,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重点项目(30230410);国家基础研究发展计划(973)项目(2006CB504705)

摘　　要：目的:观察制川乌总碱与白芍总苷、白芍多糖配伍治疗类风湿性关节炎的作用机制。方法:以制川乌总碱与白芍总苷、白芍多糖1∶2配伍治疗风寒湿证类风湿性关节炎大鼠模型,放射免疫法测定下丘脑L-ENK和β-END、血浆SP、血清IgG、血清细胞因子(IL-1β,TNF-α,IL-6,IL-2,IL-10)的含量,电镜下观察滑膜细胞超微结构的改变。结果:制川乌总碱与白芍总苷、白芍多糖1∶2配伍可以减轻风寒湿证类风湿性关节炎大鼠关节肿胀和关节疼痛,升高下丘脑L-ENK和β-END的含量,降低血浆SP、血清IgG、血清IL-1β、血清IL-6的含量,升高血清IL-2的含量,抑制成纤维样滑膜细胞分泌功能的异常亢进。结论:制川乌总碱与白芍总苷或白芍多糖配伍治疗风寒湿证类风湿性关节炎的作用机制可能是增加中枢内源性阿片肽的含量,抑制P物质合成和释放入血浆,调节血清细胞因子的紊乱,抑制血清免疫球蛋白的合成和分泌,减轻滑膜细胞异常亢进的分泌功能。Objective： To investigate the mechanism of action on compatible using of total alkaloids of Radix Aconiti Praeparata and total glycosides or polysaccharides of Radix Paeoniae Alba therapy on rheumatoid arthritis in rats. Method： The rat models of rheumatoid arthritis of cold and dampness syndrome were treated with total alkaloids of Radix Aconiti Praeparata and total glycosides or polysaccharides of Radix Paeoniae Alba. Observed the contents of hypothalamic L-ENK, hypothalamic-END, plasmatic SP, serumal IgG, serumal cell factors （IL-1β, TNF-α, IL-6, IL-2, IL-10） by radioimmunity method and ultrastructural change of synovial cell in electron microscope. Result： Total alkaloids of Radix Aconiti Praeparata and total glycosides or polysaccharides of Radix Paeoniae Alba could relieve arthrocele and arthralgia and elevate the contents of L-ENK,β-END, IL-2 and degrade the contents of SP, IgG, IL-1β, IL-6 and inhibit abnormal secretion accentuation of synovial cell like fiber. Conclusion： Total alkaloids of Radix Aconiti Praeparata and total glycosides or polysaccharides of Radix Paeoniae Alba could be used to treat rheumatoid arthritis of cold and dampness syndrome. The mechanism of action might be that the contents of center endogenous opioid peptides had increased, the synthesis and release of SP had been inhibited, the disturbance of serumal cell factor had been adjusted, and the synthesis and secretion of serum immune globulin and abnormal secretion accentuation of synovial cell had been inhibited.

关 键 词：制川乌总碱 白芍总苷 白芍多糖 配伍 类风湿性关节炎 作用机制 

分 类 号：R285.5[医药卫生—中药学]