人PrP多克隆抗体的制备及鉴定  

Preparation and Identification for the Polyclonal Antibodies of Human Mature Prion Protein

作　　者：姜海霞 毛景东[2] 王志民[1] 张学富[1] 孙乌日娜[1] 韩娜仁花[1] 

机构地区：[1]内蒙古民族大学分析测试中心,内蒙古通辽028000 [2]内蒙古民族大学动物科技学院,内蒙古通辽028000

出　　处：《动物医学进展》2009年第11期5-8,共4页Progress In Veterinary Medicine

基　　金：内蒙古民族大学硕士科研启动基金

摘　　要：制备PrP(prion protein)多克隆抗体,验证原核表达的人PrP的抗原性,为PrP空间构象的转变机制等深层次的研究以及人PrP单克隆抗体的制备提供重要的试验材料。以融合的人成熟PrP蛋白(ma-ture prion protein,mPrP)为抗原,以pET30a(+)空载体的E.coliBL21(DE3)的菌体蛋白作为对照免疫原,免疫小鼠。ELISA和Western blot分别检测多克隆抗体的效价和特异性。结果5只动物中3只均产生了较高效价的抗血清,确定人PrP多克隆抗体效价为1∶4 096,能与人mPrP特异性结合。证明获得的人mPrP具有良好的免疫原性。In order to provide experimental evidence for deep-level research of transformation mechanism of conformation of prion protein and preparation of monoclonal antibody,preparation and identification for polyclonal antibodies of human mature prion protein and validation of antigenicity of prokaryotic expression of which were carried out.The human mature prion protein by integration was used as antigen,the bacterial protein of E.coli BL21（DE3） with pET30a（＋） empty vector was used as a control immunogen to immunize mice.ELISA and Western blot were used to detect the titer and specificity of polyclonal antibodies.The result showed that three of 5 animals had resulted in anti-serum with relatively high titer,the titer of human PrP polyclonal antibodies was 1∶4 096,and able to combine with human mPrP specificly.The results certificated that the obtained human PrP had good immunogenicity.

关 键 词：朊蛋白 多克隆抗体 制备及鉴定 

分 类 号：Q511[生物学—生物化学] S852.659.7[农业科学—基础兽医学]