单管套式PCR检测外源性禽白血病病毒  

Detection of Different Subgroup of Avian leukosis viruses by Nested PCR in One Tube

作　　者：金志强 罗明星[2] 李永明[2] 汪德生[2] 史开志[2,3] 

机构地区：[1]贵州省贵阳市农业局,贵州贵阳550000 [2]贵州大学动物疫病研究所,贵州贵阳550025 [3]贵州省毕节畜牧兽医研究所,贵州毕节551700

出　　处：《动物医学进展》2009年第11期41-45,共5页Progress In Veterinary Medicine

基　　金：贵阳市农业局科学技术计划项目[2008动防-01]

摘　　要：从贵州省4个养鸡场采集出现肿瘤病变或疑似肿瘤病料样本17份,以单管套式PCR方法检测禽白血病病毒长末端重复序列(LTR),15份病料均扩增出一条213 bp的DNA带,与预期扩增长度相符合,外源性禽白血病病毒的检出率达到88.2%。同时针对禽白血病病毒的囊膜糖蛋白基因(gp85)进行PCR扩增,在被检的17份病料中,7份检出J亚群特异性的DNA带(545 bp),占41.1%;9份检出A亚群特异性的DNA带(691 bp),占52.9%;其中2份病料同时检出J亚群与A亚群特异性的病原核酸。A、J亚群的PCR检出率低于LTR的套式PCR扩增。17 affected materials were collected from 4 poultry farms in Guizhou province,which manifested tumor lesions in visceral organs.The nucleic acid of avian leukosis virus was detected against long terminal repeat（LTR） gene by nested PCR method in one tube,a 213bp DNA fragment was amplified from 15 affected materials,which was in accordance with the expected length of amplification.The detection rate of exogenous avian leukosis virus reached 88.2%.Meanwhile,PCR was conducted against envelope glycoprotein gene（gp85） of avian leukosis virus in 17 affected materials,J subgroup specific DNA band（545bp） was detected in 7 samples,accounting for 41.1%,A subgroup specific DNA band（691bp） was detected in 9 samples,accounting for 52.9%.The concurrent infections of different subgroup viruses were demonstrated because the etiological nucleic acids of J and A subgroups were detected in 2 samples.The detection rate of A and J subgroups was lower using PCR than that using the nested PCR in one tube.

关 键 词：禽白血病病毒 长末端重复序列 囊膜糖蛋白基因 单管套式PCR 

分 类 号：S858.3[农业科学—临床兽医学] Q503[农业科学—兽医学]