低氧预适应小鼠脑匀浆液提取液对大鼠鼠胚海马神经元缺氧复氧后神经细胞的影响  

Effect of brain homogenate from hypoxia-preconditioned mice on rat embryonic hippocampal neurons with hypoxia/reoxygenation injury

作　　者：杨明峰[1] 张颜波[1] 孙保亮[1] 牛敬忠[1] 吕国蔚[2] 

机构地区：[1]泰山医学院附属医院神经内科,泰安271000 [2]首都医科大学低氧医学研究所,北京100069

出　　处：《中华神经医学杂志》2009年第11期1094-1097,共4页Chinese Journal of Neuromedicine

基　　金：山东省医药卫生科技发展计划项目（2009HZ096）;山东省教育厅科研发展计划（J08LG71）;泰安市科技发展计划（20071037）

摘　　要：目的探讨低氧预适应小鼠脑匀浆液提取液对大鼠鼠胚海马神经元缺氧复氧后神经细胞活性和凋亡的影响。方法在96孔培养板中将大鼠鼠胚海马神经细胞原代培养至8d，将培养细胞按照处理的不同分为以下5组：（1）正常对照组（仅加入PBS）、（2）H风组（缺氧4h／复氧48h后加PBS）、（3）H0组（缺氧4h／复氧48h前加正常小鼠脑匀浆提取液）、（4）H1组（缺氧4h／复氧48h前加急性低氧对照小鼠脑匀浆提取液）、（5）H4组（缺氧4h／复氧48h前加低氧预适应小鼠脑匀浆提取液），分别用酶标仪和流式细胞仪测定神经细胞活性和凋亡情况。结果正常对照组细胞活性明显高于H4R48组，H0、H1和H4组分别与‰组相比，细胞活性明显增加，且H4组细胞活性又明显高于H0、H1组；正常对照组仅有极少量的凋亡细胞，而H4R48组凋亡细胞显著增多，H0、H1和H4组分别与H4R48组相比凋亡细胞明显减少，且H4组又分别明显少于H0、H1组。结论低氧预适应小鼠脑匀浆液提取液可能通过增加大鼠鼠胚海马神经元缺氧复氧后神经细胞活性和减少神经细胞凋亡起到抗缺氧性损伤作用。Objective To observe the effect of brain homogenate （BH） extracted from hypoxia-preconditioned mice on the viability and apoptosis of rat embryonic hippocampal neurons with hypoxia/reoxygenation-induced injury. Methods Rat embryonic hippocampal neurons primarily cultured for 8 days in 96 well tissue culture plate were divided into 5 groups, namely the normal control group （treated with PBS）, HnR48 group （with hypoxia for 4 h followed by reoxygenation for 48 h and PBS treatment）, H0 group （with BH from normal mice prior to hypoxia/reoxygenation）, H1 group （with BH from acute hypoxia-preconditioned mice and hypoxia/reoxygenation）, and H4 group （with BH from hypoxia-preconditioned mice and hypoxia/reoxygenation）. The viability and apoptosis of the cells in the 5 groups were observed by MTT assay and flow cytomertry, respectively. Results The cell viability was significantly higher in the normal control group than in H4R48 group. In H0, H1, and H4 groups, the cell viability increased significantly as compared with that in H4R48 group, and the cells in H4 group showed the highest viability. Apoptotic cells were scarcely observed in the normal control group, but were numerous in HaR48 group. Compared with H4R48 group, H0, H1, and H4 groups showed obviously reduced apoptotic cells, and the reduction was the most conspicuous in H4 group. Conclusion BH extracted from hypoxia-preconditioned mice may offer protection against hypoxic injury of rat embryonic hippocampal neurons challenged with hypoxia-reoxygenation by promoting the cell viability and decreasing cell apoptosis.

关 键 词：低氧预适应 缺氧复氧 细胞凋亡 海马 

分 类 号：R686[医药卫生—骨科学]