4-羟基他莫昔芬对泌乳素腺瘤GH3细胞PTTG表达的影响  

Effect of 4-hydroxytamoxifen on the expression of pituitary tumor transforming gene in GH3 prolactinoma cells

作　　者：崔友强[1] 汪建军[1] 滕良珠[2] 孔建新[1] 李猛[1] 郭建[1] 孙金龙[1] 

机构地区：[1]山东省千佛山医院神经外科,济南250014 [2]山东大学山东省立医院神经外科,济南250021

出　　处：《中华神经医学杂志》2009年第11期1103-1105,1110,共4页Chinese Journal of Neuromedicine

基　　金：山东省科技攻关项目（2006GG3202013）

摘　　要：目的探讨雌激素受体拮抗剂4-羟基他莫昔芬（OHTam）对泌乳素腺瘤GH3细胞中垂体瘤转化基因ferrc）表达的影响。方法采用逆转录多聚酶链式反应（RT—PCR）和Westem blot法，测定GH3细胞中肿GmRNA和PTTG蛋白表达。在去激素培养条件下观察不同浓度的4-羟基他莫昔芬（OHTam）和雌二醇（E2）对细胞生长速度、PTTG GmRNA和PTTG蛋白表达水平的影响。结果GH3细胞在去激素环境下细胞生长明显减慢，低浓度E2（1×10^-8mol／L）可促进其生长，OHTam（1×10^-6mol／L）可抑制E2（1×10^-8moVE）的生长刺激作用：GH3细胞中存在PTTG mRNA和PTTG蛋白的表达，且OHTam（1×10^-6mol／L）可抑制它们的表达水平。结论泌乳素腺瘤GH3细胞的生长具有雌激素依赖性，应用雌激素受体拮抗剂OHTam能抑制GH3细胞生长和PTTG原癌基因的表达水平。Objective To investigate the effect of 4-hydroxytamoxifen on the expression of pituitary tumor transforming gene （PTTG） in GH3 prolactinoma cells. Methods RT-PCR and Western blotting were employed to detect the expressions of PTTG mRNA and protein in human GH3 prolactinoma cells. Different concentrations of estradiol （E2） or 4-hydroxytamoxifen （OHTam） were addedl into the hormone-depleted medium, and the viable cell number and expression levels of PTTG mRNA and protein were measured. Results The growth of GH3 prolactinoma cells was significantly inhibited in hormone-depleted medium. E2 at a concentration of 1×10^-8 mol/L obviously promoted the cell growth, the effect of which was inhibited by the application of OHTam （1×10^-6mol/L） to cause slowed cell growth. The expressions of PTTG at both the mRNA and protein levels were detected in detected in untreated GH3 prolactinoma cells, and OHTam at the concentration of 1×10^-6 mol/L significantly inhibited their expressions. Conclusion The growth of GH3 cells is estrogen-dependant and can be inhibited by estrogen antagonist OHTam, which also results in reduced expression ofPTTG gene in the cells.

关 键 词：泌乳素腺瘤 垂体瘤转化基因 4-羟基他莫昔芬 

分 类 号：R686[医药卫生—骨科学]