以黑色素瘤抗原基因mRNA为标记检测胃癌患者外周血肿瘤细胞  

Detection of tumor cells in peripheral blood of patients with gastric cancer using mRNA of MAGE genes as markers

作　　者：王万祥[1] 李英彬[2] 谢晓亮[1] 苏秀兰[3] 欧阳晓晖[1] 

机构地区：[1]内蒙古医学院第一附属医院普通外科,呼和浩特010059 [2]内蒙古自治区医院肿瘤科 [3]内蒙古医学院第一附属医院中心实验室

出　　处：《中华胃肠外科杂志》2009年第6期611-614,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：内蒙古自治区自然科学基金（200408020931）;内蒙古自治区卫生厅项目（2005072）

摘　　要：目的以黑色素瘤抗原基因（MAGE）-1和MAGE-3mRNA作为特异性肿瘤标记物，建立用RT-PCR检测胃癌患者外周血中肿瘤细胞的有效方法。方法采集40例胃癌患者及20名健康志愿者的外周血，用RT—PCR方法检测其外周血单核细胞（PBMC）中MAGE-1和MAGE-3mRNA表达。同时用RT—PCR方法检测胃癌患者肿瘤组织中上述MAGE-1和MAGE-3的表达。结果40例胃癌患者中，分别有19例（47．5％）和10例（25．0％）PBMC中可检测到MAGE-1和MAGE-3 mRNA表达：在25例（62．5％）的胃癌患者PBMC中，至少可以检测到一种MAGEmRNA的表达。胃癌组织中MAGE-1和MAGE-3的表达率分别为62．5％和30％;胃癌组织中不表达MAGE-1和（或）MAGE-3基因的患者．其外周血中也检测不到相应的MAGE中mRNA;20例健康志愿者的PBMC中均未测出MAGE基因mRNA。胃癌患者PBMC中MAGE-1和（或）MAGE-3 mRNA的检出率与淋巴结转移和TNM分期密切相关（P〈0．05）。此外，外周血PBMC中CEA mRNA的检出率为32．5％．MAGE-1、MAGE-3和CEA互者在胃癌中联合检测的检出率为72．5％。结论MAGE—1和MAGE-3 mRNA可作为相对特异性标记物用于检测胃癌患者外周血肿瘤细胞．联合CEA可以提高其敏感性;MAGE mRNA的检测结果有助于对胃癌患者的预后进行判断。Objective To develop a sensitive and specific RT-PCR assay using the mRNA of MAGE-1 and MAGE-3 genes as specific tumor markers for the detection of the tumor cells in the peripheral blood of patients with gastric cancer. Methods Peripheral blood was obtained from 40 patients with gastric cancer and from 20 heahhy volunteers. The mRNA of MAGE-1 and MAGE-3 genes in the peripheral blood mononuclear cells （PBMC） was detected by RT-PCR. The expressions of MAGE-1 and MAGE-3 mRNA in the tumor tissues of these gastric cancer patients were also detected by RT-PCR. Meanwhile,CEA expression by nested RT-PCR in PBMC of 40 gastric cancer patients was also detected. Results Of 40 gastric cancer patients, MAGE-1 and MAGE-3 mRNA were positive in 47.5% （19/40） and 25%（10/40） of PBMC respectively, and in 62.5%（25/40） and 30%（12/40） of gastric cancer tissues respectively. As a whole, in the PBMC of 40 gastric cancer patients, 25 （62.5%） samples were found to express at least one type of MAGE mRNA. In the patients whose tumors did not express MAGE-1 and/or MAGE-3 genes, the corresponding MAGE mRNA was also undetected in their PBMC. There was no expression of MAGE-1 or MAGE-3 gene in the PBMC from the 20 healthy donors. The positive rate of MAGE mRNA in PBMC was closely correlated with the tumor stage and lymph node metastasis （P〈0.05）. Positive rate of CEA gene expression was 32.5%（13/40）in the PBMC of 40 gastric cancer patients, 29 （72.5%）samples were detected to express at least one type of MAGE gene and CEA gene mRNA. Conclusions MAGE-1, MAGE-3 and CEA mRNA are specifically detected with high percentage in the PBMC of gastric cancer patients by RT-PCR. They could be used as specific tumor markers for the detection of the circulating gastric cancer cells, and the detection results may be helpful to evaluate the prognosis of gastric cancer patients.

关 键 词：胃肿瘤 黑色素瘤抗原基因 反转录聚合酶链反应 RNA 信使 

分 类 号：R735[医药卫生—肿瘤]