Double role of Fas ligand in the apoptosis of intervertebral disc cells in vitro  

作　　者：Dunfu Han Yue Ding Shang-Li Liu Gang Wang In-Cheong Si Xinguang Wang Liyang Cui Dongshang Huang 

机构地区：[1]Department of Orthopaedic Surgery, The Second Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510120, China [2]Department of Orthopedic Surgery, The People's Hospital of Henan Province, Zhengzhou 450003, China

出　　处：《Acta Biochimica et Biophysica Sinica》2009年第11期938-947,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （No. 30471746）.

摘　　要：Fas ligand （FasL） may play an important role in maintaining the immune privilege of intervertebral disc （IVD）. Besides, it is closely related to the apoptosis of degenerative disc cells. Nowadays, lots of reports have described about the paradoxical effects of FasL, although the effect of FasL on IVD cells is still under debate. In this study, we tried to investigate the effects of FasL on Fas expression and on the apoptosis of nucleus pulposus （NP） cells in Sprague-Dawley rats. The results showed that the expression of Fas in NP cells was significantly increased by the recombinant FasL. Meanwhile, the apoptosis of NP cells increased markedly in a FasL dose-dependent manner. Interestingly, RNA interference results indicated that the increase of Fas expression and the NP cell apoptosis described previously were inhibited by Fas siRNA, suggesting that RNA interference might be one of novel strategies to prevent IVD cells from apoptosis.Fas ligand （FasL） may play an important role in maintaining the immune privilege of intervertebral disc （IVD）. Besides, it is closely related to the apoptosis of degenerative disc cells. Nowadays, lots of reports have described about the paradoxical effects of FasL, although the effect of FasL on IVD cells is still under debate. In this study, we tried to investigate the effects of FasL on Fas expression and on the apoptosis of nucleus pulposus （NP） cells in Sprague-Dawley rats. The results showed that the expression of Fas in NP cells was significantly increased by the recombinant FasL. Meanwhile, the apoptosis of NP cells increased markedly in a FasL dose-dependent manner. Interestingly, RNA interference results indicated that the increase of Fas expression and the NP cell apoptosis described previously were inhibited by Fas siRNA, suggesting that RNA interference might be one of novel strategies to prevent IVD cells from apoptosis.

关 键 词：APOPTOSIS FAS Fas ligand intervertebral disc RNA interference 

分 类 号：Q789[生物学—分子生物学] Q255