三氯乙烯对大鼠肝细胞DNA损伤和氧化应激的影响  

Effect of Trichloroethylene on DNA Damage and Oxidative Stress in Rat Hepatocytes

作　　者：彭巨成[1] 刘益民[1] 余日安[1] 吴礼康[1] 吴泰顺[1] 朱志良[1] 

机构地区：[1]广东省深圳市宝安区疾病预防控制中心,518101

出　　处：《职业与健康》2009年第24期2673-2674,共2页Occupation and Health

基　　金：深圳市宝安区科技局资助项目(编号:2005121)

摘　　要：目的研究三氯乙烯对大鼠肝细胞DNA损伤和肝组织氧化应激的作用。方法选用雄性SD大鼠,每组5只,用1500和3000 mg/kg的三氯乙烯经口灌胃染毒,48 h处死动物。用单细胞凝胶电泳(彗星实验)检测肝细胞DNA损伤,并检测肝组织中活性氧(ROS)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量。结果三氯乙烯在1500和3000 mg/kg剂量条件下,肝细胞DNA的Olive尾矩增加,DNA损伤细胞率分别为41.96%和54.13%,也明显高于对照组的9.30%(P<0.01);大鼠肝组织中的ROS、MDA均较对照组显著增高(P<0.01),同时GSH又较对照组明显下降(P<0.01)。结论三氯乙烯在1500和3000 mg/kg剂量条件下染毒大鼠后,造成肝组织明显的氧化应激并引起肝细胞DNA损伤。[ Objective ] To study the effects of triehloroethylene on DNA damage in rat hepatocytes and oxidative stress in rat liver tissue. [Methods] Male SD rats were selected, 5 for each group. Trichloroethylene at the doses of 1500 and 3000 mg/kg were poured into rats＇ stomach through mouth, then they were killed after 48 hours. DNA damage was detected by single-cell gel electro- phoresis. Contents of Reactive Oxygen Species （ ROS）, Malondialdehyde （ MDA ） and Glutathione （GSH） were also measured. [ Results ] Trichloroethylene at the doses of 1500 and 3000mg/kg caused DNA olive tail moment increased, DNA damage rate was 41.96% and 54.13%, respectively, which was significantly higher than that of control group （P 〈0.01 ） ; while GSH decreased signifantly compared with control group （P 〈 0.01）. [ Conclusion] Trichloroethylene at the doses of 1500 and 3000mg/kg induces obvious oxidative stress in rat liver tissue and causes DNA damage in rat hepatocytes.

关 键 词：三氟乙烯 氧化应激 DNA损伤 

分 类 号：R114[医药卫生—卫生毒理学]