鞘内注射依那西普对完全弗氏佐剂致痛大鼠痛阈的影响  

Effects of intrathecal etanercept on the pain threshold after complete Freund＇s adjuvant-induced inflammatory pain in rats

作　　者：文益云[1] 唐吉伟[2] 戴如萍[3] 徐军美 

机构地区：[1]长沙市湖南省肿瘤医院麻醉科,410013 [2]长沙市湖南省第二人民医院麻醉科,410001 [3]长沙市中南大学湘雅第二医院麻醇科,410000

出　　处：《实用疼痛学杂志》2009年第5期327-330,共4页Pain Clinic Journal

摘　　要：目的探讨鞘内注射依那西普（etanercept）对完全弗氏佐剂（complete Freund’s adjuvant，CFA）所致大鼠炎性疼痛的影响，进一步探讨TNF-α在炎性痛中的作用。方法SD大鼠48只，随机分为8组（n=6）。A组为腹腔注射生理盐水（NS）；B组为腹腔注射依那西普；C组为腹腔注射NS，足底注射CFA；D组为腹腔注射依那西普，足底注射CFA；E组为鞘内注射NS；F组为鞘内注射依那西普；G组为鞘内注射NS，足底注射CFA；H组为鞘内注射依那西普，足底注射CFA。测量各时间点大鼠左足50％缩足阈值。结果腹腔注射和鞘内注射etanercept均升高CFA致痛大鼠的痛闽（P〈0．05），两者间无统计学差异（P〉0．05）。结论鞘内注射依那西普升高CFA致痛大鼠的痛阈，提示TNF—α在CFA所致炎性疼痛的外周致敏和中枢致敏中均起作用。Objective To investigate the effect of intrathecal etanercept on the inflammatory pain threshold following complete Freund＇s adjuvant（CFA） in rats, and the role of TNF-α in the inflammatory pain. Methods Forty-eight Sprague-Dawley rats were randomly divided into 8 groups （n=6）. Group A： normal saline administered intraperitoneally; Group B： etanercept administered intraperitoneally; Group C： normal saline administered intraperitoneally an.d CFA injected; Group D： etanercept administered intraperitoneally and CFA injected ; Group E： normal saline administered intrathecally; Group F：etanercep administered intrathecally; Group G： normal saline administered intrathecally and CFA injected; Group H： etanercept administered intrathecally and CFA injected. 50% Paw withdrawal threshold was test at different time after injection. Results Injecting etanercept intrathecally and intraperitoneally can both elevate the pain-threshold after CFA-induced inflammatory pain in rats（P〈0.05）, and no statistical differences between them（P〉0.05）. Conclusion Injecting etanercept intrathecally can attenuate CFA-induced allodynia, which illustrates that TNF-α play a role in peripheral sensitization and central sensitization.

关 键 词：痛觉过敏 炎症 肿瘤坏死因子-α 大鼠 鞘内注射 腹腔内注射 依那西普 完全弗氏佐剂 

分 类 号：R338[医药卫生—人体生理学] R743.35[医药卫生—基础医学]