富集骨髓基质干细胞复合多孔β-磷酸三钙促进骨缺损修复的实验研究  

Repairing bone defects of goat femoral condyle by rich bone marrow stromal cells combined with porous beta-tricalcium phosphate

作　　者：翟永清[1] 干耀恺[1] 秦安[1] 戴魁戎[1] 

机构地区：[1]上海交通大学医学院附属上海市第九人民医院,200011

出　　处：《中华创伤骨科杂志》2009年第11期1058-1061,共4页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金（30801175）;上海市科委国际合作重大课题（045407008）;上海市教委课题（09YZ77）;上海市骨科内植物重点实验室建设基金（08DZ2230330）

摘　　要：目的评估富集骨髓基质干细胞（BMSCs）复合β-磷酸三钙（β-TCP）在促进骨缺损修复中的作用。方法抽取12只崇明山羊骨髓血，用梯度离心法分别将其中的BMSCs富集、分离后与β-TCP复合。12只山羊双侧股骨内髁钻孔，制备骨缺损模型。所有山羊左侧股骨内髁缺损通过富集BMSCs／β-TCP进行局部填充治疗（实验组），其中10只羊右侧股骨内髁缺损以单纯β—TCP颗粒填充治疗（对照组），2只羊右侧股骨内髁缺损旷置（空白组），评价富集效率。术后16周处死取材，将所得标本进行影像学观察和形态学计量，实验组和对照组之间进行比较。结果BMSCs富集后有核细胞数和碱性磷酸酶染色呈阳性的细胞集落形成单位（CFUs／ALP＋）数均有显著提高，差异有统计学意义（P〈0．05）。X线侧位片和μ-CT形态计量分析显示实验组新生骨长人率（70．27％±8．47％）显著高于对照组（21．40％±5．77％），差异有统计学意义（t=11．97，P〈0．05）。X线侧位片、CT平扫与三维重建以及μ-CT图像均可见实验组疗效优于对照组。结论在促进骨缺损修复中，富集BMSCs／β-TCP的疗效优于单纯β—TCP颗粒，有良好的应用前景。Objective To access the ability of rich bone marrow stromal cells combined with porous beta-tricalcium phosphate （enriched BMSCs/β-TCP） to repair large bone defects of goat femoral condyle. Methods The bone marrow was harvested from 12 Chongming goats, from which the BMSCs were isolated and condensed with density gradient centrifugation method. The enriched BMSCs were then mixed with β-TCP. Animal models of bone defects were produced by drilling holes in the femoral medial condyle in each of the 12 goats. The left side was treated with enriched BMSCs/β-TCP in 12 goats （experimental group）, while the right side with β-TCP alone （control group） in 10 goats and untreated （blank control group） in the other 2 goats. The enrichment efficiency was evaluated. After euthanasia at 16 weeks after surgery, radiological observations and morphometry were used to compare the situations between the experimental and control groups. Results After enrichment and isolation, the nucleated cells （NCs） and colony-forming units/ alkaline phosphatase ＋ （CFUs/ALP ＋ ） were increased significantly. X-ray lateral projection, CT and three-dimensional reconstruction, μ-CT images revealed that the effect of enriched BMSCs/β-TCP was better than that of β-TCP alone. X-ray lateral projection and μ-CT morphometry showed that new bone formation was higher in the experimental group. Conclusion Enriched BMSCs/β-TCP is effective in repairing bone defects and may have a promising clinical application.

关 键 词：骨髓基质干细胞 磷酸钙类 骨缺损 

分 类 号：R686[医药卫生—骨科学]