小针刀对移植于裸鼠的人皮肤增生性瘢痕组织中Ⅰ、Ⅲ型胶原蛋白的影响  

Effect of Small Needle-Knife on Collagens Ⅰ and Ⅲ in Transplanted Human Skin Hypertrophic Scar Tissues in Nude Mice

作　　者：李枫[1] 陈东明[2] 乔晋琳[3] 孙东杰[2] 唐岩[1] 唐军民[1] 赵霞[2] 毕振伍[1] 梁艳清[4] 

机构地区：[1]北京大学医学部组织胚胎学教研室,北京100191 [2]北京大学第三医院成形科,北京100191 [3]海军总医院康复理疗科,北京100037 [4]广东医学院组织胚胎教研室,广州524023

出　　处：《中国微创外科杂志》2009年第11期1038-1041,共4页Chinese Journal of Minimally Invasive Surgery

摘　　要：目的探讨小针刀对移植于裸鼠皮下人增生性瘢痕真皮组织Ⅰ、Ⅲ型胶原蛋白的作用。方法将6例人无表皮的增生性瘢痕组织分别移植于24只裸鼠背部皮下,建立增生性瘢痕裸鼠动物模型。术后10天,分为对照组、0.1mg/ml曲安奈德组、0.2mg/ml曲安奈德组和小针刀组,每组6只,14天取材,利用免疫组织化学染色检测Ⅰ、Ⅲ型胶原蛋白的变化。结果Ⅰ、Ⅲ型胶原分布于各组成纤维细胞的胞浆及组织中。图像分析结果,0.1mg/ml曲安奈德组和0.2mg/ml曲安奈德组Ⅰ、Ⅲ型胶原含量(0.09±0.03,0.11±0.05;0.12±0.02,0.11±0.01)低于对照组(0.17±0.04,0.19±0.03)(P<0.05);小针刀组Ⅰ、Ⅲ型胶原含量(0.12±0.02,0.12±0.02)与曲安奈德组差异无显著性(P>0.05)。结论小针刀可降低移植于裸鼠皮下增生性瘢痕真皮组织中Ⅰ、Ⅲ型胶原蛋白的含量。Objective To discuss the effect of small needle knife therapy on collagens Ⅰ and Ⅲ in the hypertrophic sear tissues that was subcutaneously transplanted into nude mice. Methods Six samples of human hypertrophic scar tissues without cuticle were subcutaneously transplanted into the back of 24 nude mice to create the animal models of hypertrophic scar. Ten days after of the operation, the mice were divided into control, 0. 1 mg/ml triamcinolone, 0.2 mg/ml triamcinolone, and small needle-knife groups with 6 mice in each. Specimens of the scar tissues were collected in 14 days for immunohistoehemistry to detect the collagen I and Ⅲ. Results In all the groups, collagens Ⅰ and Ⅲ distributed extensively in the cytoplasm of fibroblast and tissues. Image analysis showed that the concentration of collagens Ⅰ and Ⅲ in 0.1 mg/ml and 0.2 mg/ml triamcinolone groups were significantly lower than those in the control （0.09 ±0.03, 0. 11 ±0.05 and 0. 12 ±0.02, 0. 11±0.01 vs. 0. 17±0.04, 0. 19 ±0,03, P〈 0. 05） , but similar to that in the small needle-knife group （0.12 ± 0.02, 0.12 ± 0.02, P 〉 0.05 ）. Conclusions Small needle- knife therapy can reduce the concentration of collagens Ⅰ and Ⅲ in transplanted hypertrophic scar tissues in nude mice.

关 键 词：小针刀 裸鼠 增生性瘢痕 胶原纤维 

分 类 号：R622[医药卫生—整形外科]