人胚胎发育后期小脑皮质组织中一氧化氮合酶阳性神经元的形态学  

Morphology of Nitric Oxide Synthase Positive Neurons in the Cerebellar Cortex of Midanaphase Human Fetus

作　　者：郑兰荣[1] 黄小梅[1] 顾倩[1] 邵金贵[1] 

机构地区：[1]皖南医学院病理学教研室,安徽芜湖241002

出　　处：《中国医学科学院学报》2009年第4期473-475,I0006,共4页Acta Academiae Medicinae Sinicae

基　　金：安徽省教育厅自然科学研究基金(KJ2007B019)~~

摘　　要：目的探讨人胚胎发育后期小脑皮层内一氧化氮合酶(NOS)阳性神经元的生长、发育规律。方法采用免疫组织化学方法观察人胚胎发育后期小脑皮层组织内NOS阳性神经元的发育过程。结果第6～7个月龄时,小脑室管层内可见NOS阳性神经元细胞,核多呈圆形,细胞有短小突起。第8～9个月龄时,小脑中间层可见NOS阳性神经元细胞,核呈圆形、椭圆形或梭形,细胞体积增大,细胞质明显增多,染色加深,膨体纤维呈串珠状,伸向边缘层。第10个月龄时,边缘层变厚,主要由5～6层排列较紧密的NOS阳性神经元细胞构成,其中也有体积较小的NOS阳性神经元细胞,神经纤维变细。结论小脑内NOS阳性神经细胞产生的一氧化氮,对小脑内神经元和神经胶质细胞的分化、增殖、迁移等具有重要作用。Objective To investigate the growth and development of nitric oxide synthase （NOS）-positive neurons in the cerebellum of human fetus in the midanaphase. Method The positive expression of the NOS-positive neurons in the cerebellum of midanaphase human fetus was observed by immunohistochemistry. Results By the sixth to seventh month of gestation,NOS-positive neurons were seen in the ependymal layer of the cerebellum. The nucleus was oval-shaped and the neurons had short and small processes. By the eighth to ninth month,NOS-positive neurons were found in the central layer of the cerebellum and the nucleus was round-,oval-,or fusiform-shaped;meanwhile,the neurons grew larger in size with richer cytoplast and heavier staining. The beaded nerve fibers reached the marginal layer and the layer became thickened on the tenth month,which generally was composed of 5 to 6 layers of NOS-positive neurons that were tightly aligned. Some NOS-positive neurons were in smaller size with the cell body and the nerve fibers grew well. Conclusion Nitric oxide generated by NOS of the NOS-positive neurons in the cerebellum plays an important role in the differentiation,proliferation,and migration of neurons and gliacytes.

关 键 词：一氧化氮合酶 阳性神经元 免疫组织化学 小脑 人胚胎 

分 类 号：R338[医药卫生—人体生理学]