91例EB病毒相关疾病儿童血浆EB病毒DNA的检测  

Determination of plasma EBV DNA in 91 children with EBV-associated diseases

作　　者：段红梅[1] 姚瑶[2] 谢正德[2] 闫静[2] 胡英慧[2] 幺远[2] 周玲[3] 申昆玲[2] 

机构地区：[1]北京中医药大学护理学院,北京100102 [2]首都医科大学附属北京儿童医院,北京100045 [3]中国疾病预防控制中心病毒病预防控制所肿瘤实验室,北京100052

出　　处：《中国当代儿科杂志》2009年第11期897-900,共4页Chinese Journal of Contemporary Pediatrics

基　　金：北京市科委基金(z0005190042511);北京市教委基金(KM200710025023);北京市自然科学基金(702026)资助

摘　　要：目的了解EB病毒(EBV)感染患儿外周血血浆中游离EBVDNA的拷贝数,确定EBV原发感染后外周血血浆中EBV游离DNA的拷贝数与发病天数及病情轻重的关系。方法应用荧光定量PCR方法,测定73例EBV原发感染和18例EBV相关重症疾病患儿外周血血浆中EBV游离DNA。结果①原发EBV感染患儿外周血血浆中EBV游离DNA随发病天数呈下降趋势,发病2周后很难检测到。②EBV相关重症疾病组患儿外周血血浆中EBV游离DNA阳性率明显高于原发EBV感染组,差异有显著性(89%vs16%,P<0.05)。结论原发EBV感染后随病程天数的增加,病毒复制水平逐渐下降。血浆中EBV游离DNA检测对评价EBV相关疾病的严重程度有一定参考价值。Objective To determine the plasma level of Epstein-Barr virus （EBV） DNA in children with EBV- associated diseases, and to investigate the dynamic changes of EBV DNA level after initial infection as well as the relationship between EBV-DNA level and the diseases severity. Methods The subjects consisted of 73 children with primary EBV infection （infectious mononucleosis, pneumonia,etc. ） and 18 children with severe EBV-associated diseases （chronic active EBV infection, hemophagocytic lymphohistiocytosis, etc. ）. The plasma EBV DNA level was detected by a real-time PCR assay. Results The plasma EBV DNA level decreased with the infection time in children with primary EBV infection. Two weeks after infection, plasma EBV DNA was almost undetectable. The positive rate of plasma EBV DNA in children with severe EBV-assoeiated diseases increased significantly when compared with that in children with primary EBV infection （89% vs 16% ; P 〈0.05 ）. Conclusions The level of EBV replication may be reduced with the infection time. Dynamic determination of blood EBV DNA is useful for the evaluation of disease severity in children with EBV infection.

关 键 词：EB病毒 慢性活动性EB病毒感染 噬血细胞性淋巴组织细胞增生症 传染性单核细胞增多症 儿童 

分 类 号：R725.1[医药卫生—儿科]