脓毒症小鼠心和肾组织中巨噬细胞移动抑制因子的表达  

The expression of macrophage migration inhibitory factor in the heart and renal tissue in rats with sepsis

作　　者：张振辉[1] 林珉仪[1] 陈晓辉[1] 江慧琳[1] 李艳玲[1] 王华军[1] 

机构地区：[1]广州医学院第二附属医院危重病学科,广州510260

出　　处：《中华急诊医学杂志》2009年第11期1159-1162,共4页Chinese Journal of Emergency Medicine

摘　　要：目的 探讨巨噬细胞移动抑制因子（MIF）在脓毒症小鼠心脏和肾组织中的表达规律。方法采用盲肠结扎穿孔术（CLP）建立脓毒症BALB／c小鼠模型。30只小鼠随机分为5组，分别为假手术组，CLP后12、24、36、48h组，其中假手术组小鼠在假手术后24h取标本检测作为对照组，其余四组小鼠分别在CLP术后的12h、24h、36h和48h取标本待检。采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western Blotting）检测脓毒症小鼠心脏和肾组织中MIF的mRNA和蛋白表达。采用单因素方差分析（one—way ANOVA）方法进行计量资料的统计分析。结果与对照组比较，MIF在脓毒症小鼠心脏组织中的表达增加，在CLP术后12h开始升高（P〈0．05），36h达峰值（P〈0．01），48h仍维持较高水平（P〈0．05）；肾组织中MIFmRNA的表达在CLP术后12h开始增加（P〈0．05），24h达峰值（P〈0．01），48h后才开始减少，MIF蛋白则只在CLP术后24h和36h增加明显（P〈0．05）。结论脓毒症发生后的12h～48h，MIF在脓毒症小鼠心脏和肾组织中的表达有不同程度增加，表达时相基本一致，提示MIF可作为晚期细胞因子参与小鼠脓毒症的发病。Objective To investigate the expression profile of macrophage inhibitory factor （MIF） in heart and renal tissues of sepsis mice. Method The sepsis model was established by Cecal ligation and puncture （CLP） in mice. Thirty male BALB/c mice were randomly divided into five groups： sham operation group, the twelfth hour, twenty-forth hour, thirty-sixth and forty-eighth hour group after CLP. MIF mRNA were semiquantitated by the reverse transcription polymerase chain reaction（ RT-PCR）, Western Blotting was used for MIF protein. The measured data were analyzed with one-way ANOVA. Results MIF mRNA and protein expressions in heart tissue significantly increased at the twelfth hour, peaking at the thirty-sixth hour, and a high level was maintained till the forty-eighth hour after CLP. But in the kidney tissue of models, the content of MIF reached peak at the twenty-forth hour and started to decrease at the forty-eighth hour after CLP. Conclusions The content of MIF in heart and kidney tissues of sepsis models was higher than that in the sham group, especially from the twelfth hour to forty-eighth hour after CLP. It indicates that MIF as a kind of late cytokine might participate in dysfunction of organs in mice with sepsis.

关 键 词：巨噬细胞移动抑制因子 脓毒症 炎症 细胞因子 

分 类 号：R459.7[医药卫生—急诊医学]