谷氨酸/天冬氨酸转运体抗体对豚鼠听性脑干反应及毛细胞形态的影响  

The Effect of the Glutamate/Aspartate Transporter Antibody on Auditory Brainstem Response and Morphology of Hair Cells in Guinea Pig's Cochlea

作　　者：纪育斌[1,2] 王锦玲[1] 邱建华[1] 刘顺利[1] 

机构地区：[1]第四军医大学西京医院全军耳鼻咽喉专科中心,西安710032 [2]第二炮兵总医院耳鼻咽喉科

出　　处：《听力学及言语疾病杂志》2009年第6期566-569,共4页Journal of Audiology and Speech Pathology

摘　　要：目的研究谷氨酸/天冬氨酸转运体(glutamate-aspartate transporter,GLAST)抗体对豚鼠耳蜗听性脑干反应(ABR)和耳蜗毛细胞形态的影响。方法健康豚鼠20只随机分为实验组和对照组,每组10只。实验组耳蜗鼓阶内灌注GLAST抗体,对照组灌注人工外淋巴液,观察两组术后3、6、9天ABR反应阈、耳蜗基底膜铺片和透射电镜的形态学改变。结果实验组术后第3天ABR波形消失,术后第9天无恢复;对照组术后第3天8只动物ABR波形消失,术后第6天和第9天全部动物引出ABR波形,平均阈值分别为62.50±5.25、47.50±6.18dBSPL,差异有统计学意义(P<0.05)。随着GLAST抗体灌注后时间延长,实验组内、外毛细胞及纤毛出现不同程度缺失,透射电镜显示内、外毛细胞及神经末梢胞浆、线粒体空化,细胞核染色质边集等凋亡早期征象。对照组的损伤较轻,与ABR阈值改变相一致。结论耳蜗内GLAST抗体灌注后出现耳蜗毛细胞、神经末梢的损伤及ABR波形消失,提示GLAST抗体阻断耳蜗Corti器中的GLAST,导致谷氨酸的神经毒性表达。Objective The purpose of this investigation was to observe the effects of the glutamate/aspartate transporter（GLAST） antibody to auditory brainstem response（ABR） and the pathologic morphology of hair cells in the guinea pig＇s cochlea. Methods 20 guinea pigs were randomly divided into the experimental group and control group. By perfusing the antibody to GLAST into tympanic canal in the cochlea of guinea pigs in experimental group and artificial perilymph into the guinea＇s cochlea in control group, the results of ABR , basilar membrane stretched preparation and transmission electron microscope were observed. Results After antibody perfusion, the ABR was not induced from the third day to the ninth day. In control group, the ABR was nearly absent on the third day, but could be evolved,on the sixth day and the ninth day in all guinea pigs with the average threshold of 62. 504±5.25 dB SPI. and 47. 50±6. 18 dB SPL, respectively. The ABR thresholds at different time in control group were significantly different（P〈0.05）. Hair cells damages could be observed in experimental group after operations . The control group also expressed the damage of hair cells and reversible elevation of ABR threshold. Conclusion GLAST antibody can induce the damage of hair cell,nerve endings and auditory cilia. GLAST antibody may block GLAST functions and produce the neurotoxin of the glutamate.

关 键 词：谷氨酸/天冬氨酸转运体 耳蜗 豚鼠 

分 类 号：R764.5[医药卫生—耳鼻咽喉科]