BHC80基因抑制对斑马鱼胚胎红细胞发育的影响  

The Effects of BHC80 Down Regulation on Embryonic Erythropoiesis in Zebrafish

作　　者：侯嘉云[1] 蒋璆[1] 张洁[1] 谭理[1] 宋后燕[1] 

机构地区：[1]复旦大学分子医学教育部重点实验室,上海200032

出　　处：《生物化学与生物物理进展》2009年第11期1469-1474,共6页Progress In Biochemistry and Biophysics

摘　　要：胚胎整体RNA原位杂交显示,BHC80基因表达主要集中在中枢神经系统部位.应用吗啡啉修饰的反义寡核苷酸技术抑制BHC80基因表达,显示胚胎红细胞减少并堆积在PBI区,用胚胎期红细胞标志βe3globin以及造血过程中的重要转录因子gata1、c-myb、lmo2的胚胎整体RNA原位杂交实验显示,BHC80基因表达下调使gata1标记胚胎红系前体细胞增殖增多并且分化延迟,导致红细胞减少和PBI区红细胞堆积.血管内皮标志基因flk-1的RNA探针原位杂交和荧光显微造影显示,BHC80基因表达下调组血管与对照组相比清晰可见无明显差异.Whole mount in situ hybridization with BHC80 RNA probe showed that BHC80 was expressed in zebrafish central nervous system.Morpholino-modified antisense oligonucleotide was injected into zebrafish zygotes to knock down BHC80 expression.BHC80 knockdown resulted in striking decrease of erythrocytes and accumulation of erythrocytes at PBI.Further investigation of embryonic erythrocytes marker βe3 globin and hematopoiesis transcription factors gata1,c-myb and lmo2 by in situ hybridization showed that the erythroid progenitors marked with gata1 in BHC80 knockdown embryos were high proliferation and their differentiation were delayed,which led to decrease of erythrocytes and accumulation of erythrocytes at PBI.Both in situ hybridization and microangiography indicated that vasculature pattern of BHC80 knockdown embryos were almost normal.

关 键 词：斑马鱼 BHC80 红细胞发育 整体原位杂交 

分 类 号：Q3[生物学—遗传学] Q7