多孔碳酸化羟基磷灰石水泥修复双侧股骨髁骨缺损:同体随机对照16周组织学结果验证  

Repairing bilateral femur condular defects using porous carbonated hydroxyapatite cement:A 16 weeks result verification of homobody control experiment

作　　者：姚琦[1,2] 黄鹏[3] 唐佩福[3] 王继芳[3] 

机构地区：[1]北京大学第九临床医学院 [2]北京世纪坛医院骨科,北京市100038 [3]解放军总医院骨科,北京市100853

出　　处：《中国组织工程研究与临床康复》2009年第42期8229-8232,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金项目(39670731)资助~~

摘　　要：背景:课题组采用发泡剂成孔技术,制成了有知识产权的新型骨修复材料多孔碳酸化羟基磷灰石,既保留了碳酸化羟基磷灰石骨水泥原位固化性能等所有的优点,同时又形成多孔结构。目的:通过动物实验进一步验证制备的新型骨修复材料多孔碳酸化羟基磷灰石水泥修复骨缺损的效果。设计、时间及地点:同体对比观察实验,于2000-01/2002-08在解放军总医院骨科研究所及医学动物实验中心完成。材料:以碳酸钙、磷酸氢钙等化学试剂为原材料,通过高温烧结合成碳酸化羟基磷灰石粉体,粉体与固化液相混合原位固化形成碳酸化羟基磷灰石;在碳酸化羟基磷灰石粉体中加入成孔剂,成孔剂在骨水泥固化过程中发生化学反应产生二氧化碳气体,由此形成多孔碳酸化羟基磷灰石。方法:采用10只新西兰大白兔在双侧股骨髁制备直径为5.5mm、深12mm的骨缺损动物模型,随机选择一侧作为实验组,调和多孔碳酸化羟基磷化石,迅速将其置于特制的注射器中,注入骨缺损。另一侧为对照组,骨缺损直接填充碳酸化羟基磷化石。主要观察指标:分别于术后2,4,8,12,16周分批处死动物。通过X射线和组织学观察其修复效果。结果:实验组骨缺损逐渐被新生骨填充,骨组织逐渐改建,趋于成熟。对照组材料的边缘区有新骨生长,并随时间呈递增趋势,材料的中央区未见新骨组织。术后16周影像学检查,实验组材料与周围正常骨的密度相当,很难区分界线,对照组材料的可视面积明显减少。结论:多孔碳酸化羟基磷灰石水泥具有原位固化性能和良好的生物相容性,能作为自体骨移植的一种替代物修复骨缺损。BACKGROUND： Newly porous carbonated hydroxyapatite cement （PCHC） was prepared using foaming agent-hole technique, which possess merits of CHC with porous structures. OBJECTIVE： To verify the effective of PCHC for repairing bone defects. DESIGN, TIME AND SETTING： The homobody contrast observation was performed at the Department of Orthopedics, and animal experiment center, General Hospital of Chinese PLA between January 2000 and August 2002. MATERIALS： The calcium carbonate and calcium phosphate was combined and solidified to prepare CHC with high temperature calcinations, and then foaming agent was added to produce carbon dioxide gas to form porosity. METHODS： The animal model of bone defect was made on the bilateral femur of 10 New Zealand white rabbits, which were randomly divided into the experimental group （the defect was repaired with PCHC） and the control group （repaired with CHC）. MAIN OUTCOME MEASURES： All the animals were sacrificed under anesthesia at weeks 2, 4, 8, 12 and 16 after operation. The repair status of specimen was studied by X-ray and histological observation. RESULTS： The bone defect was completely filled by PCHC, and the interface between bone and PCHC was tightly combined with good biocompatibility. There was new bone formation in the control group, increased with time prolonged, but no new bone was found in the center of materials. The 16-week imaging showed that the PCHC would gradually degrade as the implant time prolonged and it would be substituted by new born bone. At the same time, the visual area of material in the control group was obviously reduced. CONCLUSION： PCHC has good biological compatibility, processes the character of immobilization, which can be used as bone substitute to repair the bone defects.

关 键 词：多孔碳酸化羟基磷灰石水泥 骨缺损 组织学 

分 类 号：R318[医药卫生—生物医学工程]