胰岛素样生长因子-1对胃平滑肌细胞合成干细胞因子的作用  

Effects of insulin-like growth factor-1 on expression of stem cell factor in gastric smooth muscle cells

作　　者：李慧[1] 林琳[1] 宁月季[1] 张蔚[1] 徐丽明[1] 李学良[1] 

机构地区：[1]南京医科大学第一附属医院消化科,210029

出　　处：《中华消化杂志》2009年第10期653-657,共5页Chinese Journal of Digestion

基　　金：国家重点基础研究发展计划（2006CB503908）;江苏省自然科学基金（BK2008466）

摘　　要：目的 探讨胰岛索样生长因子-1（IGF-1）对胃平滑肌细胞（SMC）及其合成的干细胞因子（SCF）的影响.方法 使用酶解法原代培养胃SMC,在进行-actin鉴定后,IGF-1组于细胞培养液中分别加入不同浓度的IGF-1（0、50、100、150ìg/L）,抗IGF-1组于细胞培养液中加入IGF-1（100μg/L）和IGF-1α受体（IGF-1Rα）抗体（浓度分别为0、50、100,150μg/L）,分别培养24 h.以四甲基偶氮唑盐（MTT）比色试验检测细胞增殖程度,以Western印迹法、逆转录（RT）-PCR、酶联免疫吸附试验（ELISA）检测细胞中及细胞上清中SCF的表达情况.结果 IGF-1可促进胃SMC增殖和SCF合成增加,离体实验中100μg/L可能是IGF-1促进胃SMC增殖和SCF合成的有效终浓度;IGF-1Rα抗体可抑制胃SMC增殖和SCF合成,抑制作用呈浓度依赖性.结论 IGF-1介导胃SMC合成SCF增加.Objective To investigate the effect of exogenous insulin-like growth factor-1 （IGF- 1 ） on growth of gastric smooth muscle cells （SMCs） and synthesis of stem cell factor （SCF）. Methods Gastric SMCs were cultured by enzymolysis and were identified by α- actin immunocytochemical method. After that, SMCs were incubated with different concentrations of IGF-1 （0, 50, 100, 150 ug/L） or IGF-1 receptor monoclonal antibody （0,50,100,150ug/L） for 24 hours. Proliferation of SMCs was measured by MTT. The expressions of SCF protein and RNA were analyzed by Western blotting and quantitative reverse transcription polymerase chain reaction, respectively. The SCF content in the culture fluid was detected by enzyme-linked immunosorbent assay （ELISA）. Results The proliferation of gastric SMCs and the expression of SCF can be enhanced by IGF-1, and 100 pg/L of IGF-1 may be the final effective concentration. Nevertheless, these could be inhibited by IGF 1 receptor monoclonal antibody in a dose-dependent manner. Conclusion Elevated expression of SCF may be induced by IGF-1 through proliferation of SMC.

关 键 词：胰岛素样生长因子1 肌细胞 平滑肌 干细胞因子 抗体 

分 类 号：R686[医药卫生—骨科学]