栉孔扇贝急性病毒性坏死病毒(AVNV)卵内的垂直传播途径  

Study on AVNV vertical transmission sensu stricto in scallop Chlamys farreri

作　　者：于佐安[1,2] 王崇明[1] 李赟[2] 任伟成[1,2] 蔡玉勇[1,2] 

机构地区：[1]中国水产科学院黄海水产研究所,山东青岛266071 [2]中国海洋大学水产学院,山东青岛266003

出　　处：《水产学报》2009年第6期1031-1036,共6页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2006AA100307);公益性行业(农业)科研专项课题(nyhyzx07-047);山东省科技攻关项目(2004GG2205115)

摘　　要：急性病毒性坏死病毒已被证实为是一种对养殖栉孔扇贝危害极大的病原体,探讨其传播途径是防控扇贝"大规模死亡症"的前提之一。将亲贝各放在一独立育苗水体内产卵、孵化,并进行幼虫培育。通过春季和秋季室内人工育苗,从40个独立育苗水体中,采获亲贝、卵各23个家系样品,受精卵、D形幼虫、壳顶幼虫各12个家系样品,眼点幼虫2个家系样品。采用间接ELISA法、间接免疫荧光技术和电子显微镜技术对亲贝、卵和发育各期的幼虫进行定性和定位的检测。结果发现,栉孔扇贝的性腺组织中存在病毒粒子,而卵、受精卵及各期发育幼虫体内均未检测到病毒。由此可以判断:AVNV可以感染栉孔扇贝的性腺,但并不存在卵内垂直传播的可能性。The virus named AVNV （acute viral necrotic virus） can cause the mass mortality in cultured scallops Chlamys farreri. So the research of AVNV vertical transmission is necessary. In the experiment,the study put the parental scallops into the separate breeding waters,then spawning,hatching,and cultivating larvae. According to the artificial spring and autumn seed of scallop indoors,from the forty separate breeding water bodies,23 parental scallops,23 eggs,12 spermoviums,D larvae and shell young,2 eyebot larvae were obtained. For the qualitative and localized detection,the methods of Indirect ELISA,IFA and Electron microscope were used to detect parental scallops,eggs and the larvae with different development phases. The result shows that the virus can be detected in the gonad of the parental scallops,but it can not be detected in the eggs,spermoviums,D larvae,shell young,and eyebot larvae. This indicates that AVNV can infect the gonad of scallop （Chlamys farreri）,but there does not exist the route of vertical transmission sensu stricto. For further study,the research of horizonatal transmission is essential.

关 键 词：AVNV 垂直传播 间接ELSIA 间接免疫荧光 电子显微镜技术 

分 类 号：S941.41[农业科学—水产养殖]