PMSF对TOCP诱导的母鸡大脑中神经丝含量的变化  

Effects of PMSF on alteration of neurofilaments proteins in cerebrum of hens in progress of OPIDN

作　　者：窦丹丹[1] 宋福永[1] 曾涛[1] 于丽华[1] 赵盛[1] 辛星[1] 谢克勤[1] 

机构地区：[1]山东大学公共卫生学院毒理学研究所,山东济南250012

出　　处：《毒理学杂志》2009年第5期337-341,共5页Journal of Toxicology

基　　金：科技部973项目(2002CB512907);中国博士后科学基金(20080431191)

摘　　要：目的研究苯甲基磺酰氟(PMSF)阻断有机磷诱导的迟发性神经病(OPIDN)母鸡大脑中神经丝(NFs)的动态变化和机制。方法成年母鸡随机分为正常对照组、磷酸三邻甲苯酯(tri-o-cresyl phosphate,TOCP)组和PMSF+TOCP组,Western-blotting检测母鸡染毒后第1、5、10和21天大脑中高分子量神经丝蛋白(NF-H)、中分子量神经丝蛋白(NF-M)、低分子量神经丝蛋白(NF-L)3种神经丝蛋白的相对含量。结果TOCP染毒后母鸡出现OPIDN症状,大脑中NFs含量明显改变,且大脑沉淀中比上清中变化更加明显。总的趋势是从第1天开始降低,第10天达到最低,第21天有所恢复。大脑沉淀中NF-L、NF-M和NF-H在第10天分别降低了86.22%(P<0.01),86.98%(P<0.01),54.05%(P<0.05)。提前24 h给予PMSF后,与正常对照相比,母鸡未出现明显OPIDN症状,大脑上清和沉淀中NF-L和NF-M均无明显变化,NF-H有所增加,在沉淀中第5天增加最多达109.05%(P<0.01)。沉淀中PMSF+TOCP组NF 3个亚单位比TOCP组在各时间点均有不同程度的增加。结论TOCP染毒引起母鸡大脑中NFs紊乱,提前给予PMSF能够抑制这种变化,提示TOCP诱发的NF紊乱可能是OPIDN发生的一种机制。Objective To investigate the dynamic changes and mechanism of neurofilament（NF） proteins in cerebrums of hens protected by phenylmethylsulfonyl fluride（PMSF）.Methods Adult Roman hens were randomly divided into there sets,i.e.control,TOCP and PMSF＋TOCP.Animals were sacrificed on d1,d5,d10,d21.The levels of high molecular neurofilament（NF-H）,medium molecular neurofilament （NF-M） and low molecular neurofilament（NF-L） in cerebrum were analyzed by western-blotting.Results The hens in TOCP set showed severe symptoms of OPIDN.The levels of NF-L,NF-M and NF-H in pellet changed more obviously than those in supernatant,which began to decrease on 1day,sharply decreased 86.22%（P〈0.01）,86.98%（P〈0.01）,54.05%（P〈0.05）respectively on day 10,and came back little on day 21.The OPIDN symptoms were not seen in PMSF＋TOCP set.Compared with control,NF-L and NF-M in PMSF＋TOCP set remained no changes,and NF-H was increased,mostly 109.05%（P〈0.01） in pellet on day 5.The levels of NFs in pellet of PMSF＋TOCP set were decreased at different degrees in contrast with those in TOCP set.Conclusion TOCP may induce imbalance of NF levels in progress of OPIDN,and PMSF pretreatment may protect animals from OPIDN by reducing above changes,which may disclose that TOCP-induced changes of NFs may be a mechanism of the occurrence and development of OPIDN.

关 键 词：磷酸三邻甲苯酯 迟发性神经病 苯甲基磺酰氟 神经丝 

分 类 号：R114[医药卫生—卫生毒理学]