内皮素-1和干细胞因子对黑素细胞肌动蛋白的影响  

Effect on Actin in Melanocytes by Endothelin-1 and Stem Cell Factor

作　　者：牟宽厚[1] 马慧群[2] 张宪旗[3] 冯捷[2] 

机构地区：[1]西安交通大学医学院第一附属医院皮肤科,陕西西安710061 [2]西安交通大学医学院第二附属医院皮肤科,陕西西安710004 [3]浙江大学附属第一医院皮肤科,浙江杭州310003

出　　处：《中国皮肤性病学杂志》2009年第11期689-691,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(30200247);陕西省科技攻关项目〔2006K12-G3(10)〕

摘　　要：目的探讨细胞因子内皮素-1(ET-1)和干细胞因子(SCF)对培养的黑素细胞肌动蛋白的作用。方法采用免疫荧光技术观察黑素细胞在受到ET-1,SCF作用后细胞肌动蛋白actin在细胞内分布情况,间接判断其对细胞行为的影响。结果黑素细胞在细胞因子的作用下,细胞肌动蛋白呈现聚集性变化,细胞活动功能增强。尤其以ET-1作用更明显(P<0.05),而actin活动功能的增加直接关系着黑素细胞的粘附和迁移。结论ET-1和SCF在体外均可促进黑素细胞肌动蛋白actin的聚集,从而间接发挥它们促进黑素细胞粘附和迁移的作用。Objective Study the effect on actin in melanocytes by cell factors like endothelin-1 （ ET-1 ） and stem cell fac tor（SCF）in vitro. Methods Human epidermal melanocytes that had been cultured and purified. ET-1 and SCF were added to the second generations of cultured melanocytes, after 24hrs the actins in melanocytes were investigated by immunofluorescence technique and image analysis. Results After intervened by cell factors, actins in melanocytes were congregated, especially by ET-1 （ P 〈 0.05 ）. Conclusion ET-1 and SCF can promote actins＇ congregation in melanocyte in vitro and then accelerate on cell＇s adhesion and migration.

关 键 词：黑素细胞 内皮素-1 干细胞因子 细胞肌动蛋白actin 

分 类 号：R392.12[医药卫生—免疫学]