甘肃棘豆生物碱部位体内抗肿瘤作用及对免疫功能的影响  

In vivo Anti-tumor Effect of Oxytropis kansuensis Alkaloid Fraction and Its Influence on Immune Function

作　　者：王明娟[1] 刘铭[1] 刘伟[1] 杨爱军[1] 王晨昱[1] 柳军玺[2] 李敏[1] 邸多隆[2] 

机构地区：[1]兰州大学基础医学院病理学研究所,兰州730000 [2]中国科学院兰州化学物理研究所天然药物重点实验室

出　　处：《中国中西医结合杂志》2009年第11期1009-1011,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：中国科学院知识创新工程重要方向项目(No.KGCX2-SW-213-08);甘肃省中医药科研项目(No.GZK-2008-1)

摘　　要：目的研究甘肃棘豆生物碱部位对KM小鼠肝癌H22细胞抑制作用及其对KM小鼠免疫功能的影响。方法采用鼠源性肝癌细胞H22移植瘤法,将60只KM小鼠随机分为5组,其中高、中、低剂量组分别给予甘肃棘豆生物碱部位32、16、8mg/kg体重灌胃,阴性对照组给予生理盐水(NS)20mL/kg灌胃,5-氟脲嘧啶(5-FU)组给予5-FU15mg/kg体重腹腔注射,每天1次,连续给药10天。检测平均瘤重和抑瘤率,计算脾指数、胸腺指数,MTT法测脾细胞增殖率。结果甘肃棘豆生物碱部位高、中、低剂量组对肝癌细胞H22实体移植瘤的抑制率分别为43%、29%、23%;各剂量组小鼠的脾指数和胸腺指数均明显高于阴性对照组,中、高剂量组明显高于5-FU组(P<0·05);各剂量组在24、48、72h的脾细胞增殖率也明显高于阴性对照组及5-FU组(P<0·01)。结论甘肃棘豆生物碱部位对KM小鼠肝癌H22移植瘤有抑制作用,并能增强KM小鼠免疫功能。Objective To study the in vivo anti-tumor effect of Oxytropis kansuensis alkaloid fraction OKAF） in live cancer cell line H22 and its influence on immune function in KM mice. Methods Sixty mice with transplanted mouse originated liver cancer cell line H22 were divided randomly into five groups, the three OKAF groups were administrated with OKAF in low （8 mg/kg）, moderate （16 mg/kg） and high （32 mg/kg） dosage, the negative control group was administrated with normal saline 20 mL/kg and the positive control with 5-FU 15 mg/kg, respectively, once a day for 10 successive days. Then the average tumor weight （TW）, tumor inhibition rate （TIR）, spleen index （SI）, and thymus index （TI） in them were calculated, and the spleen cell proliferation rate （SCPR） was measured by MTT assay. Results TIR of low, moderate and high dosage of OKAF was 23%, 29% and 43% respectively. SI and TI in all the three OKAF groups were higher than those in the negative control group, and the two indexes in the moderate and ligh dosage OKAF groups were higher than those in the positive control group （ P 〈 0.05）. SCPR in OKAF groups at 24 h, 48 h and 72 h were higher than those in both two control groups at the same time. Conclusion OKAF shows inhibitory action on liver cancer cell line H22 tumor in KM mice, and it could also enhance the immune function of mice.

关 键 词：甘肃棘豆 生物碱部位 KM小鼠 H22 细胞 抑瘤率 免疫功能 

分 类 号：S859.87[农业科学—临床兽医学]