T细胞淋巴瘤1和CD44蛋白在Burkitt淋巴瘤中的表达及其诊断价值  

Immunohistochemical study using T-cell lymphoma antibody 1 and CD44 in diagnosis of Burkitt＇s lymphoma

作　　者：水若鸿[1] 陆洪芬[1] 朱雄增[1] 

机构地区：[1]复旦大学附属肿瘤医院病理科复旦大学上海医学院肿瘤学系,200032

出　　处：《中华病理学杂志》2009年第11期745-748,共4页Chinese Journal of Pathology

基　　金：基金项目：上海市卫生局科研课题资助项目（2007Y50）

摘　　要：目的探讨T细胞淋巴瘤1（TCL1）和CD44蛋白在Burkitt淋巴瘤中的表达及其诊断价值。方法在石蜡包埋的实验组25例Burkitt淋巴瘤和对照组25例非特指弥漫性大B细胞淋巴瘤（DLBCL）中采用免疫组织化学EnVision法检测CD44、TCL1以及CD10、bcl-2、bcl-6、c—myc、Ki-67等常用抗体表达情况。结果Burkitt淋巴瘤中瘤细胞96％（24例）呈TCL1阳性，仅4％（1例）CD44阳性；88％（22例）CD10阳性、92％（23例）bcl-6和c—myc阳性，仅4％（1例）bcl-2阳性；Ki-67增殖指数为95％～100％。非特指DLBCL中仅16％（4例）TCL1弱阳性，84％（21例）CD44阳性、32％（8例）CD10阳性、72％（18例）bcl-6和bcl-2阳性、c—myc均阴性，Ki-67增殖指数40％～90％。结论当形态和免疫表型不典型时，TCL1和CD44两种蛋白的检测有助于提高Burkitt淋巴瘤的确诊率及其与DLBCL的鉴别诊断。Objective To study the values of immunohistochemistry using T-cell lymphoma antibody （TCL） 1 and CD44 in the diagnosis of Burkitt＇s lymphoma. Methods Immunohistochemical study for TCL1, CD44, CD10, bel-2, bel-6, e-myc and Ki-67 was performed on paraffin-embedded sections of lymphoma cases, including 25 cases of Burkitt＇s lymphoma and 25 cases of diffuse large B-cell lymphoma. Results Burkitt＇s lymphoma commonly expressed TCL1 （96% ,24 cases）, CD10 （88% ,22 cases）, bcl-6 and c-myc （92% ,23 cases）. Only 1 case（4% ） expressed CD44 and bcl-2. The Ki-67 proliferation index ranged from 95% to 100%. On the other hand, diffuse large B-cell lymphoma expressed CIM4（84% ,21 cases）, CD10（32%,8 cases）, bcl-6（72%,18 cases） and bcl-2（72%,18 cases）. Four cases（16%） were weakly positive for TCL1. The staining for c-myc was all negative. The Ki-67 proliferation index ranged from 40% to 90%. Conclusion Immunohistoehemical staining for TCL1 and CD44 is a useful ancillary tool in the pathologic diagnosis of Burkitt＇s lymphoma which is also helpful for the differential diagnosis from diffuse large B-cell lymphoma.

关 键 词：伯基特淋巴瘤 淋巴瘤 B-细胞 抗原 CD44 诊断 鉴别 抗体 TCL 

分 类 号：R686[医药卫生—骨科学]