冠心病合并2型糖尿病患者血清MMP-9,TIMP-1及hs-CRP水平的检测及其临床意义  

Detection of MMP-9,TIMP-1 and hs-CRP in coronary heart disease patients with type 2 diabetes mellitus and the clinical significance

作　　者：章惺惺[1] 蔡雪黎[2] 汪大望[1] 

机构地区：[1]温州医学院附属第一医院内分泌科,浙江温州325000 [2]温州医学院附属第一医院心血管内科,浙江温州325000

出　　处：《温州医学院学报》2009年第5期439-442,共4页Journal of Wenzhou Medical College

摘　　要：目的:探讨外周静脉血清基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制剂-1(TIMP-1)、高敏C反应蛋白(hs-CRP)水平与冠心病(CHD)合并2型糖尿病(T2DM)的关系并评价其临床意义。方法:选取CHD合并T2DM患者31例,单纯CHD患者50例,选择门诊体检者30例为正常对照组。用酶联免疫吸附法(ELISA)检测血清MMP-9,TIMP-1,用免疫散射比浊法检测血清hs-CRP的表达情况。结果:CHD合并T2DM组血清MMP-9的浓度(M=409.62ng/mL)显著高于CHD组(M=263.40ng/mL)及对照组[(196.15±44.89)ng/mL(]均P<0.05)。CHD合并T2DM组血清TIMP-1的浓度[(229.27±46.85)ng/mL]低于对照组[(272.75±72.35)ng/mL(]P<0.05)。CHD合并T2DM组血清hs-CRP的浓度(M=17.20mg/L)明显高于CHD组(M=4.57mg/L)及对照组[(1.52±0.78)mg/L(]均P<0.01)。CHD合并T2DM组患者血清MMP-9,TIMP-1与hs-CRP的浓度呈正相关。结论:联合检测血清MMP-9、TIMP-1与hs-CRP可作为一种更为有效的预测糖尿病冠状动脉粥样硬化发生的临床指标。Objective： To explore the effect of matrix metalloproteinase-9 （MMP-9）,tissue inhibitor of metalloproteinase-1 （TIMP-1） and high sensitive C-reactive protein （hs-CRP） in coronary heart diseas（eCHD）with type 2 diabetes mellitus （T2DM）. Methods： Thirty one CHD with T2DM and 50 patiens with CHD were chosen;and 30 healthy volunteers served as control group. MMP-9 and TIMP- 1 in serum were measured with ELISA, hs-CRP in serum was measured by immunoturbidimetric method. Results： The level of MMP-9 in CHD with T2DM （M=409.62 ng/mL） was significantly higher than that of CHD group （M=263.40 ng/mL）（P〈0.05）and the control group[（196.15±44.89）ng/mL（]P〈0.05）. The level of TIMP-1 in CHD with T2DM [（229.27±46.85）ng/mL] was significantly lower than that of control group [（272.75±72.35）ng/mL（]P〈0.05）. The level of hs-CRP in CHD with T2DM（M=17.20 mg/L） was significantly higher than that of CHD group （M=4.57 mg/L（）P 〈0.01）and that of the control group[（1.52±0.78）mg/ L（]P〈0.01）. MMP-9 and TIMP-1 were significantly related with hs-CRP in CHD with T2DM （[r =0.482, P〈0.01）（r =0.458,P〈0.05）]. Conclusion： Combined detection of serum MMP-9,TIMP-1 and hs-CRP can be used as effective clinical parameters forecasting diabetes related coronary atherosclerosis.

关 键 词：基质金属蛋白酶 基质金属蛋白酶组织抑制剂 高敏C反应蛋白 冠心病 糖尿病 2型 

分 类 号：R587.1[医药卫生—内分泌]