中国儿童A族链球菌感染菌株emm分型及超抗原基因谱分布  被引量:10

Emm types and superantigen analysis of streptococcus pyogenes isolated from Chinese children

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作  者:马耀玲[1] 杨永弘[1] 俞桑洁[1] 姚开虎[1] 袁林[1] 沈叙庄[1] 

机构地区:[1]首都医科大学北京儿童医院儿科研究所微生物及免疫室,北京100045

出  处:《基础医学与临床》2009年第11期1166-1169,共4页Basic and Clinical Medicine

基  金:国家863项目(2006AA02Z417)

摘  要:目的研究A族β溶血性链球菌感染菌(GAS)株emm分型及其超抗原在我国儿童中的分布情况。方法用聚合酶链反应(PCR)联合测序对2005年~2006年中国5家儿童医院的222株A族β溶血性链球菌感染的临床菌株进行emm分型;PCR检测speA,speC,speH,speI,speG,speJ,ssa和SMEZ超抗原基因的分布情况。结果共检测到9个emm型,emm12为主占55.86%(124/222),其次emm1占39.64%(88/222);78.39%的A族溶血性链球菌(GAS)分离株携带有6个或以上的超抗原基因,emm型和超抗原基因谱之间存在密切联系。结论中国儿童GAS感染流行菌株具有emm型分布集中和超抗原基因携带率高的特点,不同的emm分型有不同的超抗原基因谱,这将为我国GAS疫苗的研制提供重要的分子流行病学依据。Objective To investigate the distribution of the emm types and superantigens of group A streptococcal (GAS) isolated from Chinese children. Methods Totally 222 GAS isolates collected from five Children's Hospitals of China during 2005 - 2006 were studied, emm types were performed by PCR and sequencing. The eight superantigen (SAg) genes (speA, speC, spell, speI, speG, speJ, ssa and SMEZ) were checked by PCR. Results Nine emm types were identified,of which emm12 (55.86%) and emm1 (39.64%) were the most prevalent types. The GAS isolates carried six or more SAg genes take 78.39% of all the isolates in this study. The SAg gene profiles were closely associated with the emm type. Conclusion The emm type of S. pyogenes isolated from Chinese children was quite wide-spreading and SAg genes appeared to be associated with the emm type so its expression is potential in vaccine development.

关 键 词:A族Β溶血性链球菌 emm基因分型 超抗原 

分 类 号:R378[医药卫生—病原生物学]

 

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