乙酰半胱氨酸对小鼠缺血再灌注损伤肝肺组织的保护作用及机制  

Protective effect of N-acetylcysteine on liver and lung in mice after ischemia-reperfusion injury

作　　者：曾山[1] 林羿[1] 狄镜芳[1] 冯铮[1] 

机构地区：[1]暨南大学组织移植与免疫中心,广东广州510632

出　　处：《细胞与分子免疫学杂志》2009年第11期1058-1060,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30672231)

摘　　要：目的:研究抗氧化剂N-乙酰半胱氨酸(NAC)对小鼠肝脏缺血再灌注损伤模型中肝肺组织的保护作用及机制。方法:制备BALB/c小鼠肝部分缺血再灌注损伤模型,将小鼠随机分为3组:假手术组(SH组),缺血再灌注组(I/R组)和NAC组(I/R-NAC组)。分别于再灌注后1 h、3 h取静脉血测定血清TNF-α浓度和ALT水平;取肺组织标本测湿干质量比及病理。同时取肝、肺组织行RT-PCR以观测Toll样受体2/4(TLR2/4)表达。结果:肺组织病理结果显示I/R组肺组织毛细血管充血,肺泡结构破坏,肺泡间质中性粒细胞浸润,肺泡腔内不同程度渗出及少量红细胞。肺湿干质量比显示肺水肿。缺血再灌注后静脉血清TNF-α浓度和ALT水平较假手术组显著升高。受损肝叶和肺组织内均出现TLR2/4 mR-NA高表达,NAC干预后,肺水肿明显减轻;TLR2/4 mRNA表达受到抑制;血清TNF-α浓度和ALT水平较I/R组明显下降。结论:N-乙酰半胱氨酸抑制再灌注后TLR2/4的活化,降低TNF-α的分泌,从而减轻缺血再灌注肝、肺组织的损伤。AIM： To investigate protective effect of N- acetylcysteine （NAC） on liver and lung in mice after hepatic ischemia/reperfusion injury. METHODS： BALB/c mice were used in a model of partial hepatic ischemia/reperfusion （I/R） injury. They are divided randomly to sham-operated control group（SH）, hepatic I/R group or NAC pretreated in hepatic I/R group（I/R-NAC）. The level of TNF-α in protal vein and plasma ALT were measured at 1hour and 3 hour, respectively after reperfusion. Lung tissue wet-to-dry（W/D） weight ratio compared. RESULTS： Lung tissue W/D ratio showed significant difference between two groups; The expressions of TLR2/4 mRNA in liver and lung increased obvi- ously after hepatic I/R injury. Histological evaluation showed several changes in lung tissue in I/R group. The level of TNF-α and ALT in protal vein increased continually in I/R group at 1hour and 3 hour of reputation compared with SH group. The level of TNF-α and ALT declined significantly in the group pretreated by NAC. CONCLUSION： N- acetylcysteine can inhibit the activation of TLR2/4 and reduce TNF-α secretion resulted from I/R injury it might abate liver and lung injury following partial hepatic ischemia-reperfusion in mice.

关 键 词：乙酰半胱氨酸 缺血再灌注 肺损伤 

分 类 号：R446.6[医药卫生—诊断学]