活血散结中药外用治疗老年乳腺增生病模型大鼠的疗效研究  

Therapeutic effect of Huoxuesanjie external use for aged hyperplasia of mammary gland model rat

作　　者：马民[1] 张桂娟[2] 马义[3] 李德辉[1] 郭慧[1] 翟贺宁[1] 吕志刚[1] 

机构地区：[1]暨南大学医学院中医系,广东广州510632 [2]暨南大学附属第一医院 [3]暨南大学生物工程研究所

出　　处：《中国老年学杂志》2009年第21期2739-2742,共4页Chinese Journal of Gerontology

基　　金：广东省自然科学基金项目(5300544);广东省科技攻关项目(2006B35602009);广东省中医药管理局项目(1060114;2008092)

摘　　要：目的研究活血散结中药外用治疗乳腺增生病的作用机制。方法60只老年大鼠随机分为对照组、模型组、散结乳癖组、三苯氧胺组、活血散结中药小、中、大剂量组。通过雌孕激素联合造模方法,形成老年乳腺增生病模型鼠,造模同时给予不同药物进行干预。观察治疗前后模型鼠体重、血液流变学、乳房血流动力学、左侧第2只乳头高度及直径、血清激素等方面变化。结果大鼠经造模并用药干预后,第2周内出现模型组及各用药组大鼠体重增长幅度较对照组明显降低(P<0.01),第3周后恢复正常。大鼠乳腺微循环灌注量,模型组和各用药组低于对照组(P<0.01);经药物干预治疗后,各用药组微循环灌注量高于模型组(P<0.01),活血散结中药中、大剂量组高于散结乳癖组(P<0.05)。活血散结中药外用能够影响模型大鼠的血液流变学参数,改善血液的高黏高凝状态。各药物组均能明显抑制雌孕激素造模所引起的大鼠乳头高度的变化(P<0.01),活血散结中药大、中剂量组效果优于小剂量组和散结乳癖组(P<0.05)。除活血散结中药小剂量组外,其余各药物干预组均能明显抑制雌孕激素造模所引起的大鼠乳头直径的变化(P<0.01),三苯氧胺组疗效优于其他各组(P<0.05);活血散结中药中剂量组和散结乳癖组相比有明显差异(P<0.05)。活血散结中药能显著降低模型大鼠的雌激素(E2)、泌乳素(PRL)、卵泡生成素(FSH)水平,升高孕激素(P)、血清睾酮(T)和黄体生成素(LH)水平(P<0.05)。各药物均能显著降低模型大鼠乳腺增生的程度(P<0.01),活血散结中药大剂量组优于散结乳癖组(P<0.05),与三苯氧胺组无显著性差异(P>0.05)。结论活血散结中药组方外用可改善乳房部血液循环,抑制腺体增生,促进纤维组织吸收,有效调整体内激素水平,对乳腺增生病有良好疗效。Objective To investigate the mechanism of Huoxuesanjie external use for hyperplasia of mammary gland. Methods 60 aged rats were randomly divided into control, model, Sanjierubi emplastrum, tamoxifen paste, Huoxuesanjie traditional Chinese estraderm elements extracting solution small, middle and large dose groups. Hyperplasia mammary gland model rats were made by the union of estrogen and progestational hormone. At the same time of making model, the rats were given different durg. The body weight, hemorheology, breast hemodynamic, height and diameter of the left second ripple and serum hormone level were detected before and after treatment. Results Compared with control group, the body weight increasing degree decreased in model and each treatment groups on second Week（ P 〈 0. 01 ） and regain the normal level on the third week. The mammary gland circulation perfusion in control group was higher than that of model and each treatment groups （P 〈0.01 ）. After drug interference, the mammary gland circulation perfusion in each treatment group was higher than that of model group （P 〈 0. 01 ）, and the mammary gland circulation peIfusion in Huoxuesanjie middle and large dose group was higher than that of sanjierubi group（P 〈0. 05）. Huoxuesanjie could improve the model rat＇s blood high viscosity and high-coagulate state. The change of ripple height in each treatment group could be inhibited （ P 〈0. 01 ） and the effect of large and middle dose of Huoxuesanjie were better than that of small dose of Huoxuesanjie and sanjierubi （ P 〈 0. 05 ）. Except small dose of Huoxuesanjie, the other drug could inhibite the change of ripple diameter （P 〈 0. 01）, and tamoxifen was better than other drugs and there was obvious difference between middle Huoxuesanjie and sanjierubi （P 〈 0. 05 ）. Huoxuesanjie could efficiently decreased the level of estrogen, prolactin （PRL） , follicle-stimulating hormone （FSH） and increase the level of progestational hormone, testosterone, and luteinizin

关 键 词：活血散结中药外治 乳腺增生病 

分 类 号：R2-031[医药卫生—中西医结合]