传染性支气管炎病毒S_1基因DNA免疫质粒的构建及其免疫原性  

Construction and Preliminary Immunogenicity Assess of Infectious Bronchitis Virus S 1 Gene DNA Vaccine Plasmid

作　　者：步志高[1] 江国托[1] 王秀荣[1] 康丽娟[1] 祁贤 陈万芳 卢景良[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室

出　　处：《中国兽医学报》1998年第6期527-530,共4页Chinese Journal of Veterinary Science

基　　金：国家攀登计划B类项目

摘　　要：为探讨ＤＮＡ免疫防制传染性支气管炎（ＩＢ）的可能性，以克隆的Ｍａｓｓｃｈｕｓｅｔｅ型类Ｍ４１地方分离ＱＤ株Ｓ１基因为免疫原基因，引入终止密码后插入ＳＶ４０启动子、增强子下游和ＳＶ４０ｐｏｌｙＡ信号上游，构建了Ｓ１基因ＤＮＡ免疫表达质粒ｐＳＶＱＤＳ１。将大量扩增并经聚乙二醇纯化的ｐＳＶＱＤＳ１溶于ＰＢＳ，以３００μｇ／只的剂量肌肉注射免疫小鼠，于４周后采集分离免疫小鼠血清进行鸡胚病毒中和试验。结果表明，ｐＳＶＱＤＳ１能够诱导产生针对传染性支气管炎病毒（ＩＢＶ）Ｍ４１株的中和抗体，具有良好的免疫原性。The experiments were carried out to assess the probability of DNA vaccine in controlling IB. cDNA of S 1 gene were transferred from pUCQDS 1 to pSY538 for add end code TAA at its 3′ end. After that, the fragment containing intact open read frame of S 1 gene were inserted into pSV β LacZ at down stream of SV 40 enhancer and promoter and up stream of SV 40 poly A signal to replace the LacZ gene. This plasmid was designed as S 1 gene DNA vaccine expressive plasmid, pSVQDS 1. The grown and purified pSVQDS 1 were soluted in PBS (pH7.4) to inject into quadriceps muscle of mice at a dose of 300 μg. Four weeks post injection, the serum of immuned mice were collected to neutralize M 41 IBV on SPF chicken embryo. The virus neutralization test demonstrated that the S 1 gene DNA vaccine plasmid pSVQDS 1 can induce the neutralizing antibodies in mice.

关 键 词：传染性 支气管炎病毒 S1基因 DAN免疫 家畜 

分 类 号：S852.65[农业科学—基础兽医学]