猪粪和土霉素对不同肥力土壤微生物数量及活性的影响  

Effects of pig manure and oxytetracycline on microbial number and activity in soils with different fertility levels

作　　者：章明奎[1,2] 刘兆云[1,2] 

机构地区：[1]浙江大学环境与资源学院土水资源与环境研究所,杭州310029 [2]浙江省亚热带土壤与植物营养重点研究实验室,杭州310029

出　　处：《应用生态学报》2009年第11期2790-2798,共9页Chinese Journal of Applied Ecology

基　　金：教育部博士学科点专项科研基金项目(20060335018)资助

摘　　要：在不施肥和施用猪粪两种情况下,采用培养试验研究了不同浓度土霉素污染（0、0.1、1、10、100和1000 mg.kg-1）对土壤细菌丰度、酶活性和NO3--N浓度等的影响.试验培养温度为25℃,培养时间为30 d,取样分析时间分别为1、4和30 d.结果表明：在不施肥条件下,土霉素污染对土壤细菌数量及微生物活性的影响较小,土壤S1、S2和S3细菌数量、呼吸强度、酶活性和NO3--N浓度下降10%时土霉素的剂量（EC10）分别为36~1000 mg.kg-1、20~1000 mg.kg-1和4~1000 mg.kg-1;而在施用猪粪的情况下,对应的数值分别为2~656 mg.kg-1、2~81 mg.kg-1和1~42 mg.kg-1.添加土霉素对土壤细菌及酶活性的影响随土壤肥力的提高而增大,且其对土壤细菌数量和呼吸强度的影响大于对酶活性和NO3--N浓度的影响.土霉素污染对土壤微生物数量和活性的影响随时间变化而变化,一般在培养4 d时的影响最为明显.土霉素对土壤微生物的影响总体上表现为抑制作用.An incubation test was conducted to study the effects of antibiotics oxytetracycline on the microbial number and activity in three soils varied with fertility level under the conditions with or without pig manure application. The soils were injected with six concentrations （0, 0. 1, 1, 10, 100, and 1000 mg · kg^-1） oxytetracycline, and incubated for 30 d at 25℃. Soil samples were collected on the 1 st, 4th, and 30th day of incubation to measure the bacterial number, respiratory activity, and enzyme activity. Under no pig manure application, oxytetracycline had little effects on the soil bacterial number and activity. The ECl0 values of oxytetracycline based on a 10% decrement of bacterial number, respiratory activity, enzyme activity, and NO3 - -N concentration in treatment without pig manure were 36-1000 mg · kg^-1 , 20-1000 mg · kg^-1 , and g-1000 mg · kg^-1 for S1, S2 and $3, while those in treatment with pig manure application were 2-656 mg · kg^-1 , 2- 81 mg · kg^-1, and 1-42 mg · kg^-1 for S1, S2 and S3, respectively. Soil fertility level had obvious effects on the dose-response relations between oxytetracycline and soil microbial number and activity. The effects of oxytetracycline on soil microbial number and activity increased with increasing soil fertility level, and the effects on microbial number and respiratory activity were higher than those on enzyme activity and NO3 --N concentration. The effects of oxytetracycline on soil microbial number and activity were time-depending, being the greatest on the 4th day of incubation. Overall speaking, oxytetracycline could exert temporary inhibition on soil microbes.

关 键 词：土霉素 土壤微生物活性 有机肥 土壤肥力 

分 类 号：S154.3[农业科学—土壤学]