L-ficolin对A型H1N1流感病毒感染小鼠的保护作用  

Protective Effect of L-Ficolin on A/H1N1 Influenza Virus Infected Mice

作　　者：潘勤[1,2] 侯炜[3] Victor Tunje Jeza 章晓联[1,2] 

机构地区：[1]武汉大学医学院免疫学系,湖北武汉430071 [2]武汉大学医学院病毒学国家重点实验室,湖北武汉430071 [3]武汉大学医学院病毒学研究所,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2009年第6期701-704,709,F0002,共6页Medical Journal of Wuhan University

基　　金：国家重点基础研究发展973计划项目(编号:301170567);国家自然科学基金重点项目(编号:20532020);国家自然科学基金项目(编号:30670098);国家自然科学基金青年项目(编号:30800038)

摘　　要：目的:探讨L-ficolin分子与A型H1N1流感病毒的相互作用机制。方法:将pcDNA3.1-L-ficolin真核表达质粒以及对照质粒分别肌注小鼠后感染A型H1N1流感病毒,观察感染小鼠的体重变化、肺内病毒血凝滴度,并通过肺指数以及肺组织HE染色判断感染小鼠的肺部病变情况,ELISA检测肺组织IFN-γ和IL-4的水平。结果:A型H1N1流感病毒感染后,与对照组相比,注射了pcDNA3.1-L-ficolin质粒的小鼠体重无明显下降,肺内病毒血凝滴度较低,肺组织病变较轻,并且肺内IFN-γ和IL-4的水平增高。结论:pcDNA3.1-L-ficolin对小鼠抵御A型H1N1流感病毒感染有一定的保护作用,该质粒可作为抗流感病毒的新型候选制剂。Objective：To explore the role of L-ficolin against A/H1N1 influenza virus infection in vivo. Methods：pcDNA3.1-L-ficolin eukaryotic expression vector and control vector were administrated into mouse quadriceps femoris muscle by electrophoration respectively. Then the administrated mice were infected with the A/H1N1 influenza virus. The body weights of infected mice and viral hemagglutination titers in lung were detected. The degree of infected mouse lung pathology was determined by lung weight index and histopathologic analysis. IFN-γ and IL-4 production in lung tissue homogenates were determined by ELISA （enzyme-linked immunosorbent assay）. Results：After A/H1N1 influenza virus infection,the mice administrated with pcDNA3.1-L-ficolin DNA vector had no body weight loss and lower viral hemagglutination titers and presented less severe lung pathology compared with the control groups. pcDNA3.1-L-ficolin DNA vector administration in mice induced to increase IL-4 production in lung after influenza virus infection. Conclusion：pcDNA3.1-L-ficolin DNA vector can inhibit the A/H1N1 influenza virus infection in vivo. The pcDNA3.1-L-ficolin DNA vector can be a new candidate agent against A/H1N1 influenza potentially.

关 键 词：纤维胶凝蛋白 A型H1N1流感病毒 感染 

分 类 号：R392.12[医药卫生—免疫学]